神经元分化的蛋白水解控制-猫眼课题宝

神经元分化的蛋白水解控制

批准号：

6655695

负责人：

James I Kim

金额：

$4.81万

依托单位：

UNIVERSITY OF CALIFORNIA LOS ANGELES

依托单位国家：

美国

项目类别：

财政年份：

2002

资助国家：

美国

项目状态：

未结题

起止时间：

2002-05-15 至

来源：

https://reporter.nih.gov/project-details/6655695

关键词：

cell differentiation gene deletion mutation gene expression gene targeting genetically modified animals in situ hybridization laboratory mouse molecular site neurogenesis northern blottings phenotype pluripotent stem cells postdoctoral investigator protease inhibitor proteolysis

中文摘要

翻译

我们发现了一种可分泌的可溶性蛋白，能够抑制爪蟾胚胎的初级神经发生。该蛋白含有394个氨基酸，包含三个Kunitz类丝氨酸蛋白酶抑制剂结构域。这种新蛋白被命名为Trinein，并在非洲爪蟾卵中存在的母体编码mrna的差异筛选中分离出来。引人注目的是，向爪蟾胚胎中微量注射Trinein mRNA似乎对中胚层（以Xbra、sonic hedgehog、chordin为标志）和神经板（以Sox-2为标志）细胞分化几乎没有影响；然而，最终成熟神经元（神经微管蛋白和神经原素阳性）的分化在神经板中被阻断（未发表的数据）。该建议建立在这些功能数据的基础上，以了解其在哺乳动物神经发生中的功能。

英文摘要

We have discovered a secreted, soluble protein that is able to inhibit primary neurogenesis in Xenopus embryos. This 394 amino acid protein contains three serine proteinase inhibitor domains of the Kunitz class. This novel protein has been named Trinein and was isolated during a differential screen for maternally-encoded mRNAs present in the Xenopus laevis egg. Strikingly, microinjection of Trinein mRNA into Xenopus embryos seems to have little effect on mesodermal (marked by Xbra, sonic hedgehog, chordin) and neural plate (marked by Sox-2) cell differentiation; however, differentiation of definitive mature neurons (positive for neurotubulin and neurogenin) is blocked in the neural plate (unpublished data). This proposal builds upon these functional data of Trinein to understand its function in mammalian neurogenesis.

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