STRUCTURE/FUNCTION OF ZONA PELLUCIDA GLYCOPROTEINS

透明带糖蛋白的结构/功能

• 批准号: 6387803
• 负责人: DAULAT R TULSIANI
• 金额: $ 18.33万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2003-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6387803
• 关键词: cell cell interaction; egg /ovum; fertilization; high performance liquid chromatography; laboratory mouse; laboratory rabbit; oligosaccharides; protein structure function; sperm; surface antigens; zona pellucida glycoproteins

DESCRIPTION (Adapted from the Investigator's Abstract): This project focuses on the structure and function of the oligosaccharide moieties of the mouse zona pellucida (mZP) and how this extracellular glycocalyx that surrounds the egg interacts with complementary molecules on the surface of spermatozoa. Previous evidence strongly suggests that the sperm binding activity of mZP is associated with its oligosaccharide portion. Recent studies in the applicant's laboratory have demonstrated for the first time the presence of N-linked polylactosaminyl glycans and an O-linked trisaccharide that are thought to have important roles in sperm-egg recognition and binding. To assess the bioactivity of these oligosaccharides, the proposal has five specific aims: (1) to purify mZP2 and mZP3, and then cleave and radiolabel the N-linked glycans and O-linked trisaccharide, (2) to assess the functional role of the radiolabeled N-linked and O-linked glycans in sperm-egg recognition and binding, (3) to isolate and chemically characterize functional N-linked glycan(s), (4) to synthesize the O-linked trisaccharide, examine its function, and the mechanism underlying the formation of the sperm surface antigen-trisaccharide complex, and (5) to identify and chemically characterize the mouse sperm plasma membrane (PM) proteins recognized by the N-linked and/or O-linked glycans. When completed, this study will be the first published report on structure-function diversity of both N-linked and O-linked oligosaccharides. After the complementary molecules (i.e., the bioactive glycans on ZP glycoproteins and complementary receptors on sperm PM) are identified in the selected strain of experimental mice, it will be possible to address the question of interspecies and intraspecies relationships during interactions of opposite gametes. Collectively, these studies will further our understanding of the mechanism of sperm-egg interaction and fertilization in the mouse, and they will provide needed information for the regulation of the fertilization processes in other species, including humans.

描述（改编自研究者摘要）：本项目

项目成果

The biological and functional significance of the sperm acrosome and acrosomal enzymes in mammalian fertilization.

• DOI: 10.1006/excr.1998.3943
• 发表时间: 1998-05
• 期刊: Experimental cell research
• 影响因子: 3.7
• 作者: D. Tulsiani;A. Abou-haila;C. Loeser;Ben M.J. Pereira

Rat sperm surface mannosidase is first expressed on the plasma membrane of testicular germ cells.

大鼠精子表面甘露糖苷酶首先在睾丸生殖细胞的质膜上表达。

• DOI: 10.1095/biolreprod59.6.1288
• 发表时间: 1998
• 期刊: Biology of reproduction
• 影响因子: 3.6
• 作者: Pereira,BM;Abou-Haila,A;Tulsiani,DR
• 通讯作者: Tulsiani,DR

Importance of male fertility control in family planning.

男性生育控制在计划生育中的重要性。

• DOI: 10.2174/1871530314666140320112912
• 发表时间: 2014
• 期刊: Endocrine, metabolic & immune disorders drug targets
• 作者: Tulsiani,DaulatRP;Abou-Haila,Aida
• 通讯作者: Abou-Haila,Aida

Structural analysis of the asparagine-linked glycan units of the ZP2 and ZP3 glycoproteins from mouse zona pellucida.

小鼠透明带 ZP2 和 ZP3 糖蛋白的天冬酰胺连接聚糖单元的结构分析。

• DOI: 10.1006/abbi.2000.2038
• 发表时间: 2000
• 期刊: Archives of biochemistry and biophysics
• 影响因子: 3.9
• 作者: Tulsiani,DR

Characterization and immunolocalization of beta-D-glucuronidase in mouse testicular germ cells and spermatozoa.

小鼠睾丸生殖细胞和精子中 β-D-葡萄糖醛酸酶的表征和免疫定位。

• DOI: 10.1006/excr.1998.4355
• 发表时间: 1999
• 期刊: Experimental cell research.
• 作者: Abou-Haila,A;Fouquet,JP;Tulsiani,DR
• 通讯作者: Tulsiani,DR