EXPRESSION AND IN VIVO ROLE OF TROPHININ IN MICE

肌钙蛋白在小鼠中的表达和体内作用

• 批准号: 6343185
• 负责人: Michiko Fukuda
• 金额: $ 31.03万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6343185
• 关键词: cell adhesion molecules; cell line; embryo implantation; gene expression; gene targeting; genetically modified animals; human tissue; laboratory mouse; membrane proteins; protein structure function; transcription factor; western blottings; yeast two hybrid system

DESCRIPTION (adapted from investigator's abstract): The goal of this proposal is to define the in vivo role of trophinin in embryo implantation using the mouse as a model. The specific aims are: 1) to define the in vivo role of trophinin by analyzing trophinin gene knockout mice; 2) to define the expression and detailed localization of trophinin in mouse blastocysts and endometrium by determining the localization of trophinin in mouse blastocyst and endometrial epithelium at peri-implantation periods by immunoelectron microscopy, 3) to define the interaction between trophinin and tastin by yeast two-hybrid assays, and 4) to define the promoter activity of the human trophinin gene in order to understand the temporal and spatial expression of the trophinin in the cells involved in implantation.

描述（改编自研究者摘要）：本研究的目标 一项建议是确定滋养蛋白在胚胎着床中的体内作用 用老鼠做模型。 具体目标是：1）确定在 通过分析trophinin基因敲除小鼠的trophinin的体内作用; 2） 目的：明确滋养蛋白在小鼠体内的表达和定位 通过确定滋养层蛋白在子宫内膜中的定位， 小鼠胚泡和子宫内膜上皮在围着床期， 免疫电镜，3）以确定营养素之间的相互作用 和tastin，以及4）确定启动子 活性的人营养素基因，以了解时间和 滋养蛋白在参与着床的细胞中的空间表达。