EXPRESSION AND IN VIVO ROLE OF TROPHININ IN MICE

描述(改编自调查人员摘要)：本报告的目标 建议确定体内营养素在胚胎着床中的作用 使用鼠标作为模型。具体目标是：1)定义In 通过分析营养素基因敲除小鼠的体内作用；2) 确定营养素在小鼠体内的表达和详细定位 胚泡和子宫内膜中滋养素定位的研究 小鼠胚泡和围植入期子宫内膜上皮细胞 免疫电子显微镜，3)确定营养素之间的相互作用 和tastin，以及4)确定启动子 人营养素基因的活性，以了解时间和 营养素在着床相关细胞中的空间表达。

DESCRIPTION (adapted from investigator's abstract): The goal of this proposal is to define the in vivo role of trophinin in embryo implantation using the mouse as a model. The specific aims are: 1) to define the in vivo role of trophinin by analyzing trophinin gene knockout mice; 2) to define the expression and detailed localization of trophinin in mouse blastocysts and endometrium by determining the localization of trophinin in mouse blastocyst and endometrial epithelium at peri-implantation periods by immunoelectron microscopy, 3) to define the interaction between trophinin and tastin by yeast two-hybrid assays, and 4) to define the promoter activity of the human trophinin gene in order to understand the temporal and spatial expression of the trophinin in the cells involved in implantation.