RESEARCH CENTER FOR CEREBRAL VASCULAR DISEASE

脑血管疾病研究中心

• 批准号: 6382432
• 负责人: MYRON DAVID GINSBERG
• 金额: $ 7.5万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-08-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6382432
• 关键词: cerebral ischemia /hypoxia; neuroprotectants

The objective of the Cerebral Vascular Disease Research Center at the University of Miami is to carry out an integrated, multi-disciplinary program of experimental research into the mechanisms and therapies of ischemic brain injury. The overall goal of our research program is to identify and characterize those factors responsible for engendering ischemic injury that may be amenable to therapeutic or prophylactic intervention. The Program Project consists of three investigative proposals supported by General-Scientific and Image- Analysis/Computing Core facilities. Project areas include 1) focal cerebral ischemia: a study of the mechanisms of neuroprotection underlying human serum albumin therapy, and of exacerbation of injury due to hyperthermia (fever); 2) mitochondria and cerebral anoxia/ischemia: basic mechanistic studies of protection and dysfunction; and 3) the metabolic and structural consequences of evolving mitochondrial and ionic dysfunction in focal ischemia. The projects are closely integrated with one another, both conceptually and methodologically, and are strongly supported by close collaborative interactions among the investigators and research personnel. The core facilities contribute substantively to the implementation of each project and unify the overall program by common methodological approaches. The General Scientific Core compromises animal physiology; methods of focal and global cerebral ischemia; neurobehavioral strategies; morphological methods including quantitative light microscopy and stereology; immunohistochemistry, confocal microscopy; autoradiographic strategies for local cerebral blood flow and glucose utilization; enzymatic tissue sampling and metabolic analyses; and molecular biological methods, including in situ hybridization, Northern and Western blotting, and reverse transcription-polymerase chain reaction (RT-PCR). The Image-Analysis and Computing Core has pioneered in the development of three-dimensional autoradiographic image-averaging and is a central resource supporting image-analysis of autoradiographic, molecular biological, and histopathological data; as well as acquisition, statistical analysis and archiving of laboratory data, instrumentation control, and data base management.

迈阿密大学脑血管疾病研究中心的目标是开展一项综合性、多学科的实验研究计划，研究缺血性脑损伤的机制和治疗方法。我们的研究计划的总体目标是确定和表征那些负责产生缺血性损伤的因素，这些因素可能适用于治疗或预防性干预。该计划项目包括三个由一般科学和图像分析/计算核心设施支持的调查建议。项目领域包括1）局灶性脑缺血：研究人血清白蛋白治疗的神经保护机制，以及高温（发热）导致的损伤加重; 2）线粒体和脑缺氧/缺血：保护和功能障碍的基本机制研究; 3）局灶性缺血中线粒体和离子功能障碍的代谢和结构后果。这些项目在概念上和方法上彼此紧密结合，并得到调查人员和研究人员之间密切合作互动的有力支持。核心设施为每个项目的实施做出了实质性贡献，并通过共同的方法统一了整个方案。一般科学核心包括动物生理学;局灶性和全脑缺血的方法;神经行为策略;形态学方法，包括定量光学显微镜和体视学;免疫组织化学，共聚焦显微镜;局部脑血流和葡萄糖利用的放射自显影策略;酶组织取样和代谢分析;以及分子生物学方法，包括原位杂交、北方和西方印迹以及逆转录聚合酶链反应（RT-PCR）。图像分析和计算核心率先开发了三维放射自显影图像平均，是支持放射自显影，分子生物学和组织病理学数据的图像分析的中央资源;以及实验室数据的采集，统计分析和存档，仪器控制和数据库管理。