ENDOGENOUS ANTIBIOTICS IN NASAL LAVAGE

这一提议背后的基本假设是，囊性纤维化患者气道表面液体中内源性抗生素水平降低。 因此，具体目标是从患有和不患有CF的患者获得鼻分泌物。 将测量分泌物中乳铁蛋白和溶菌酶的水平，并将其标准化为体积、蛋白质和粘蛋白含量。 气道粘膜下腺的浆液细胞富含乳铁蛋白、溶菌酶等多种天然抗生素。 CF中浆液细胞的液体分泌大大减少。 囊性纤维化中分泌性囊泡的运输改变也可能减少到达气道表面的抗生素的量。 这将有助于假单胞菌和其他病原体在囊性纤维化中的气道定植，这是这种疾病的特征。

The underlying hypothesis behind this proposal is that levels of endogenous antibiotics in airway surface liquid are reduced in cystic fibrosis. Accordingly, the specific aim is to obtain nasal secretions from patients with and without CF. Levels of lactoferrin and lysozyme in the secretions will be measured and normalized to volume, protein and mucin content. Serous cells of airway submucosal glands are rich in a variety of natural antibiotics such as lactoferrin and lysozyme. Fluid secretion by serous cells is greatly reduced in CF. It is also possible that altered trafficking of secretory vesicals in cystic fibrosis reduce the amounts of antibiotics reaching the airway surface. This would contribute to the colonization of airways in cystic fibrosis by pseudomonas and other pathogens, which is characteristic of this disease.