ALTERATIONS IN AIRWAY SURFACE LIQUID IN CYSTIC FIBROSIS
ALTERATIONS IN AIRWAY SURFACE LIQUID IN CYSTIC FIBROSIS
批准号:
6527149
负责人:
Jonathan H Widdicombe
金额:
$95.43万
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-09-01 至 2003-08-31
中文摘要
提出的SCOR将检验两个基本假设来解释cf相关
英文摘要
The proposed SCOR will test two basic hypothesis to explain the CF-related
modifications in airway surface liquid (ASL) that lead to colonization by
Pseudomonas. The serous cell malfunction hypothesis proposes that serous
cells in the submucosal glands of large airways and on the surface of
small airways show reduced secretion of fluid and antibiotics in CF. The
resulting decreased levels of serous cell antibiotics and increased mucin
concentrations in ASL favor pathogen colonization. The high salt
hypothesis proposes that human airway epithelium absorbs Na and Cl, that
a substantial fraction of Cl absorption occurs by an transcellular route,
and that block of this route by malfunction of CFTR promotes higher than
normal NaCl content of ASL in CF. A less likely possibility is that
elevated NaCl levels in CF ASL are caused by saltier than normal gland
secretions resulting from failure of ductal salt absorption. High salt
content of ASL may encourage Pseudomonas colonization by inhibiting the
action of natural antibiotics. Project 1 (Widdicombe/Bastacky) will use
low-temperature scanning electron microscopy and X-ray microanalysis of
rapidly frozen tissues to determine how the regulation of ASL depth is
altered in CF. Salt and mucin content of ASL and gland changes in depth,
composition and viscosity of ASL in living tissues using novel
fluorescence microscopy. Project 3 (Wine) will study the secretory
mechanisms contributing to the ASL, and test specifically the serous cell
malfunction hypothesis. Project 4 (Miller) will determine the routes and
mechanisms by which Na and Cl are absorbed across airway surface
epithelium. Nasal PD measurements will be used to verify in vitro
findings. A Cell Culture Core (Finkbeiner) will provide intact human
airways and cultures of human gland and surface epithelial cells.
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Estimate of the subepithelial hydrostatic pressure that drives inflammatory transudate into the airway lumen.
估计将炎症渗出液推入气道腔的上皮下静水压。
DOI:
10.1152/japplphysiol.00645.2001
发表时间:
2002
期刊:
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子:
--
作者:
[Serikov,VB, Jang,YJ, Widdicombe,JH]
通讯作者:
Widdicombe,JH
Distribution of tracheal and laryngeal mucous glands in some rodents and the rabbit.
一些啮齿动物和兔子的气管和喉粘液腺的分布。
DOI:
10.1046/j.1469-7580.2001.19820207.x
发表时间:
2001
期刊:
Journal of anatomy
影响因子:
2.4
作者:
[Widdicombe,JH, Chen,LL, Sporer,H, Choi,HK, Pecson,IS, Bastacky,SJ]
通讯作者:
Bastacky,SJ
Altered NaCl concentration of airway surface liquid in cystic fibrosis.
改变囊性纤维化气道表面液体的氯化钠浓度。
DOI:
10.1007/s004240100636
发表时间:
2001
期刊:
Pflugers Archiv : European journal of physiology.
影响因子:
--
作者:
[Widdicombe,JH]
通讯作者:
Widdicombe,JH
Electrophysiological approach to studying CFTR.
研究 CFTR 的电生理方法。
DOI:
10.1385/1-59259-187-6:49
发表时间:
2002
期刊:
Methods in molecular medicine.
影响因子:
--
作者:
[Fischer,Horst]
通讯作者:
Fischer,Horst
DOI:
10.2746/042516402776180151
发表时间:
2002
期刊:
Equine veterinary journal
影响因子:
2.2
作者:
[Widdicombe,JH, Pecson,IS]
通讯作者:
Pecson,IS
共 6 条
LOW TEMPERATURE SEM AND X RAY MICROANALYSIS OF ASL
-
批准号:6354737
-
项目类别:
-
资助金额:$18.09万
-
财政年份:2000
-
负责人:Jonathan H Widdicombe
-
依托单位:
ENDOGENOUS ANTIBIOTICS IN NASAL LAVAGE
-
批准号:6308609
-
项目类别:
-
资助金额:$1.75万
-
财政年份:1999
-
负责人:Jonathan H Widdicombe
-
依托单位:
LOW TEMPERATURE SEM AND X RAY MICROANALYSIS OF ASL
-
批准号:6202599
-
项目类别:
-
资助金额:$18.09万
-
财政年份:1999
-
负责人:Jonathan H Widdicombe
-
依托单位:
ALTERATIONS IN AIRWAY SURFACE LIQUID IN CYSTIC FIBROSIS
-
批准号:6389917
-
项目类别:
-
资助金额:$94.67万
-
财政年份:1998
-
负责人:Jonathan H Widdicombe
-
依托单位:
ALTERATIONS IN AIRWAY SURFACE LIQUID IN CYSTIC FIBROSIS
-
批准号:6056520
-
项目类别:
-
资助金额:$89.48万
-
财政年份:1998
-
负责人:Jonathan H Widdicombe
-
依托单位:
ALTERATIONS IN AIRWAY SURFACE LIQUID IN CYSTIC FIBROSIS
-
批准号:2621743
-
项目类别:
-
资助金额:$90.46万
-
财政年份:1998
-
负责人:Jonathan H Widdicombe
-
依托单位:
ALTERATIONS IN AIRWAY SURFACE LIQUID IN CYSTIC FIBROSIS
-
批准号:6184475
-
项目类别:
-
资助金额:$92.04万
-
财政年份:1998
-
负责人:Jonathan H Widdicombe
-
依托单位:
LOW TEMPERATURE SEM AND X RAY MICROANALYSIS OF ASL
-
批准号:6110946
-
项目类别:
-
资助金额:$18.09万
-
财政年份:1998
-
负责人:Jonathan H Widdicombe
-
依托单位:
REGULATION OF RESPIRATORY TRACT FLUID LAYERS
-
批准号:6109984
-
项目类别:
-
资助金额:$11.7万
-
财政年份:1997
-
负责人:Jonathan H Widdicombe
-
依托单位:
CORE--CELL BIOLOGY LABORATORY
-
批准号:6105620
-
项目类别:
-
资助金额:$5.2万
-
财政年份:1997
-
负责人:Jonathan H Widdicombe
-
依托单位:
CFTR AND AIRWAY PATHOLOGY OF CYSTIC FIBROSIS
-
批准号:2220436
-
项目类别:
-
资助金额:$51.08万
-
财政年份:1993
-
负责人:Jonathan H Widdicombe
-
依托单位:
CFTR AND AIRWAY PATHOLOGY OF CYSTIC FIBROSIS
-
批准号:2028450
-
项目类别:
-
资助金额:$60.03万
-
财政年份:1993
-
负责人:Jonathan H Widdicombe
-
依托单位:
CFTR AND AIRWAY PATHOLOGY OF CYSTIC FIBROSIS
-
批准号:3106855
-
项目类别:
-
资助金额:$51.0万
-
财政年份:1993
-
负责人:Jonathan H Widdicombe
-
依托单位:
CFTR AND AIRWAY PATHOLOGY OF CYSTIC FIBROSIS
-
批准号:2519306
-
项目类别:
-
资助金额:$58.51万
-
财政年份:1993
-
负责人:Jonathan H Widdicombe
-
依托单位:
CFTR AND AIRWAY PATHOLOGY OF CYSTIC FIBROSIS
-
批准号:2220435
-
项目类别:
-
资助金额:$50.08万
-
财政年份:1993
-
负责人:Jonathan H Widdicombe
-
依托单位:
PROTEIN PHOSPHORYLATION AND AIRWAY CHLORIDE SECRETION
-
批准号:3239604
-
项目类别:
-
资助金额:$10.52万
-
财政年份:1988
-
负责人:Jonathan H Widdicombe
-
依托单位:
REGULATION OF APICAL MEMBRANE CL CHANNELS IN AIRWAYS
-
批准号:3106856
-
项目类别:
-
资助金额:$67.93万
-
财政年份:1988
-
负责人:Jonathan H Widdicombe
-
依托单位:
REGULATION OF APICAL MEMBRANE CL CHANNELS IN AIRWAYS
-
批准号:3106859
-
项目类别:
-
资助金额:$59.84万
-
财政年份:1988
-
负责人:Jonathan H Widdicombe
-
依托单位:
PROTEIN PHOSPHORYLATION AND AIRWAY CHLORIDE SECRETION
-
批准号:3239603
-
项目类别:
-
资助金额:$8.89万
-
财政年份:1988
-
负责人:Jonathan H Widdicombe
-
依托单位:
PROTEIN PHOSPHORYLATION AND AIRWAY CHLORIDE SECRETION
-
批准号:3239602
-
项目类别:
-
资助金额:$6.78万
-
财政年份:1988
-
负责人:Jonathan H Widdicombe
-
依托单位:
海外基金