MMP EXPRESSION IN A NOVEL IN VITRO WOUND HEALING MODEL

新型体外伤口愈合模型中的 MMP 表达

• 批准号: 6379881
• 负责人: NADEEM Y KARIMBUX
• 金额: $ 4.33万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-15 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6379881
• 关键词: angiogenesis; collagenase; epithelium; gingiva; histology; human tissue; immunocytochemistry; immunofluorescence technique; tissue /cell culture; western blottings; wound healing

The objective for this Small Grant Application is to test the hypothesis, "angiogenisis and epithelialization in periodontal wounds are mediated by membrane-type matrix metalloproteinase-1 (MT-MMP-1) and gelatinase A in a time and tissue specific manner." It has three Aims. In Aim 1, an in vitro gingival wound healing model will be established using surgical specimens of human gingiva. The specimens will be obtained from routine graft procedures, second stage implant or periodontal flap surgeries, cored, and epithelium removed to generate a 3-mm wound. The wounded tissue will be cultured for 0, 3 5 and 7 days and processed for histology, immuohistochemistry and Western blotting. Culture medium will be examined by zymography. In Aim 2, cryosections will be subjected to histology and immunofluorescence with several chosen antibodies. The extent of reepithelialization will be quantified by histology, and proliferative activity of keratinocytes measured by BrdU uptake. In Aim 3 expression of MT-MMP-1 and gelatinase A will be examined by immunofluorescence with anti-MT-MMP-1 and MMP-2 antibodies, Western analysis of wound extracts, and gelatin zymography of culture medium.

这项小额拨款申请的目的是测试假设，