CORE--MOLECULAR BIOLOGY

核心--分子生物学

• 批准号: 6459008
• 负责人: HARVEY D PREISLER
• 金额: $ 31.28万
• 依托单位: RUSH UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-07 至 2003-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6459008
• 关键词: biomedical facility; cytokine; gene expression; molecular biology; tumor suppressor genes

The function of the molecular biology core is to provide the methodologies and analyses required by Projects I and III. This Core will be responsible for assessment of expression of IRF1/IRF2; RB, p53 and and various cytokines; detection of mutations in RAS, p53 and fms genes; analyzing the conformation changes of p53 protein and rb phosphorylation status and their relationship to cytokine production; and performing the telomerase activity and clonality assays.

分子生物学核心的功能是提供 项目一和项目三所需的方法和分析。 这一核心 将负责评估IRF 1/IRF 2的表达; RB， p53和各种细胞因子;检测RAS、p53 分析p53蛋白的构象变化， Rb磷酸化状态及其与细胞因子产生的关系; 并进行端粒酶活性和克隆性测定。