CLIENT: CLinic-based Infection Examination through Nucleic acid Technology

客户：通过核酸技术进行临床感染检查

• 批准号: TS/I000666/1
• 负责人: Tom Brown
• 金额: $ 49.96万
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=TS%2FI000666%2F1
• 关键词: CLIENT; CLinic; based; Infection; Examination

The paradigm for the diagnosis of sexually transmitted infections (STI analysis) currently involves utilising the sensitivity and specificity achieved through nucleic acid detection methodologies (detecting the DNA or RNA from the pathogen). This requires sending clinical samples to remote laboratory facilities which have the required sophisticated and expensive instrumentation. The ideal innovation in this field is to enable diagnosis and treatment ('test and treat') within a single visit to the clinic whilst the patient briefly waits for the diagnosis. In the CLIENT project we plan to transform this old paradigm by implementing an innovative combination of novel science and technology to deliver a simple to use, rapid, inexpensive, Point-of-Care (POC) analysis system for STIs. The project will achieve this by bringing together 4 different creative contributions involving:i)the latest fluorescent colour chemistries which are compatible with the probe-based detection technology to be deployed (HyBeacon probes), especially chemistries with low background noise to greatly improve detection sensitivity for positive clinical samples.ii) the most adaptable of probe technologies already trialed in direct sampling studies of chlamydia (and gonnorhoea) from urine and swabs in PCR reactions, and separately proven to function in the necessarily faster unithermal amplification methods that will be used.iii) a proven inexpensive desktop amplification and fluorescence detection instrument that is compatible with the above HyBeacon probe technology which will be further developed to analyse multiple infective agents simultaneously (multiplex analysis).iv) an expert STI research and clinical department that is currently testing thousands of samples a year.The collaborators in this project are all based in the UK, the underlying technology was invented in the UK and the key background IP is owned by the industrial partners on this project.The industry partners have sales routes to market already established or in hand.

目前诊断性传播感染的范例(性传播感染分析)涉及利用通过核酸检测方法(检测病原体的DNA或RNA)获得的敏感性和特异性。这需要将临床样本送到偏远的实验室设施，那里有所需的精密和昂贵的仪器。这一领域的理想创新是在患者短暂等待诊断的同时，能够在一次到诊所就诊的情况下进行诊断和治疗(“测试和治疗”)。在客户项目中，我们计划通过实施创新的科学和技术组合来改变这种旧的模式，以提供简单易用、快速、廉价的性传播感染护理点(POC)分析系统。该项目将通过汇集4种不同的创造性贡献来实现这一点，涉及：i)与将要部署的基于探针的检测技术兼容的最新荧光颜色化学试剂(HyBeacon探针)，特别是低背景噪声的化学试剂，以极大地提高对阳性临床样本的检测灵敏度。ii)已在聚合酶链式反应反应中对尿液和拭子中的衣原体(和革兰氏菌)进行直接采样研究的最具适应性的探针技术，Iii)与上述HyBeacon探针技术兼容的经验证的廉价桌面扩增和荧光检测仪器，该仪器将被进一步开发以同时分析多个感染性病原体(多重分析)。iv)目前每年测试数千个样本的专家STI研究和临床部门。该项目的合作者都在英国，基础技术是在英国发明的，关键的背景知识产权由该项目的行业合作伙伴拥有。行业合作伙伴已经建立了进入市场的销售路线。