Laser Spectroscopy of the Cornea

角膜激光光谱检查

• 批准号: 6325333
• 负责人: Roy S Chuck
• 金额: $ 16.98万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6325333
• 关键词: biomarker; biomedical equipment development; biotechnology; burns; computer assisted diagnosis; cornea; diagnosis quality /standard; fluorescence; fluorescence spectrometry; histopathology; human tissue; laboratory rabbit; laser spectrometry; organ culture; refractive keratoplasty; technology /technique development; time resolved data; tissue engineering

DESCRIPTION: (Applicant's Abstract) Dr. Roy Chuck is an M.D., Ph.D. who obtained his degrees from Columbia University under auspices of the National Institutes of Health's prestigious Medical Scientist Training Program Prior to that he obtained an Engineering Science degree from the University of California al Berkeley. He possesses a unique background that encompasses the fields of laser spectroscopy, biochemistry and ophthalmology. The long term objective of this application is to characterize the spectroscopic properties of the cornea during ultraviolet laser ablation to allow identification of spectral features that ma3 differentiate normal from diseased tissue. Specifically, this will be accomplished by the measurement of laser-induced fluorescence in real-time during 193-nm excimer laser corneal surger3 in various normal and disease states including chemical burns and diabetes. All of the data taken together, besides enhancing our knowledge of the spectral features of the cornea in various disease states of ocular health, may also result in the bioengineering development of an instrument to guide the alteration of excimer laser ablative patterns to achieve more predictable surgical results by using real-time spectroscopic feedback information. Much of the work will be accomplished at the Beckman Laser Institute at the University of California, Irvine which is well equipped to support the in vivo and clinical studies of excimer laser corneal surgery and vision within this research program. The Beckman Laser Institute which is located quite close to the department offices is closely affiliated with the Department of Ophthalmology. Housed within the Beckman Laser Institute is a clinical excimer laser that will be used for this study (VISX Star2). In addition, the Beckman Laser Institute contains a fully equipped vivarium for housing of rabbits, the experimental animal that will be used in these investigations Core histology and ultrastructure facilities are also available within the institute. The UCI Department of Ophthalmology has an active cornea and external disease service. This clinical practice will provide the patients for the clinical studies in the final phase of this research.

罗伊·查克博士是医学博士、博士 在国立大学的赞助下从哥伦比亚大学获得学位 卫生研究院享有盛名的医学科学家培训计划 他从加州大学获得了工程科学学位 加州大学伯克利分校。他拥有独特的背景，包括 激光光谱学、生物化学和眼科学领域。 这个应用程序的长期目标是描述 紫外线激光消融术中角膜的光谱特性 允许识别MA3与正常区分的光谱特征 病变组织。具体地说，这将通过测量 193 nm准分子激光角膜激光实时诱导荧光 外科医生3处于各种正常和疾病状态，包括化学烧伤和 糖尿病。 所有的数据放在一起，除了增强我们对光谱的知识 眼部健康的各种疾病状态下的角膜特征，也可以 导致了生物工程仪器的开发，以引导 改变准分子激光消融模式以达到更可预测的效果 通过使用实时光谱反馈信息获得手术结果。 大部分工作将在贝克曼激光研究所完成 加州大学欧文分校，装备精良，支持体内 以及准分子激光角膜手术和视力的临床研究 研究计划。贝克曼激光研究所，距离 该部门的办公室与该部有密切的联系。 眼科。贝克曼激光研究所内有一台临床准分子 这项研究将使用的激光(Visx Star2)。此外，贝克曼 激光研究所包含一个装备齐全的隔膜，用于安置兔子， 将用于这些研究的实验动物核心组织学 研究所内也有超微结构设施。 UCI眼科有活跃的角膜和外部疾病 服务。这一临床实践将为临床患者提供 在本研究的最后阶段进行研究。