Laser Spectroscopy of the Cornea

角膜激光光谱检查

• 批准号: 6951137
• 负责人: Roy S Chuck
• 金额: $ 19万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6951137
• 关键词: biomarker; biomedical equipment development; biotechnology; burns; computer assisted diagnosis; cornea; diagnosis quality /standard; fluorescence; fluorescence spectrometry; histopathology; human tissue; laboratory rabbit; laser spectrometry; organ culture; refractive keratoplasty; technology /technique development; time resolved data; tissue engineering

DESCRIPTION: (Applicant's Abstract) Dr. Roy Chuck is an M.D., Ph.D. who obtained his degrees from Columbia University under auspices of the National Institutes of Health's prestigious Medical Scientist Training Program Prior to that he obtained an Engineering Science degree from the University of California al Berkeley. He possesses a unique background that encompasses the fields of laser spectroscopy, biochemistry and ophthalmology. The long term objective of this application is to characterize the spectroscopic properties of the cornea during ultraviolet laser ablation to allow identification of spectral features that ma3 differentiate normal from diseased tissue. Specifically, this will be accomplished by the measurement of laser-induced fluorescence in real-time during 193-nm excimer laser corneal surger3 in various normal and disease states including chemical burns and diabetes. All of the data taken together, besides enhancing our knowledge of the spectral features of the cornea in various disease states of ocular health, may also result in the bioengineering development of an instrument to guide the alteration of excimer laser ablative patterns to achieve more predictable surgical results by using real-time spectroscopic feedback information. Much of the work will be accomplished at the Beckman Laser Institute at the University of California, Irvine which is well equipped to support the in vivo and clinical studies of excimer laser corneal surgery and vision within this research program. The Beckman Laser Institute which is located quite close to the department offices is closely affiliated with the Department of Ophthalmology. Housed within the Beckman Laser Institute is a clinical excimer laser that will be used for this study (VISX Star2). In addition, the Beckman Laser Institute contains a fully equipped vivarium for housing of rabbits, the experimental animal that will be used in these investigations Core histology and ultrastructure facilities are also available within the institute. The UCI Department of Ophthalmology has an active cornea and external disease service. This clinical practice will provide the patients for the clinical studies in the final phase of this research.

描述：（申请人的摘要）Roy Chuck博士是医学博士，博士谁 在国家科学院的赞助下，他获得了哥伦比亚大学的学位。 卫生研究院著名的医学科学家培训计划之前， 他在纽约大学获得了工程科学学位， 加州伯克利。他拥有独特的背景， 激光光谱学、生物化学和眼科学领域。 本申请的长期目标是表征 在紫外激光消融过程中角膜的光谱特性， 允许识别区分正常和异常的光谱特征 病变组织具体而言，这将通过测量 193nm准分子激光角膜术中实时激光诱导荧光 外科医生3处于各种正常和疾病状态，包括化学烧伤， 糖尿病 所有的数据加在一起，除了增强我们对光谱的了解外， 在眼睛健康的各种疾病状态下的角膜特征也可以 导致生物工程开发的仪器，以指导 改变准分子激光烧蚀图案以实现更可预测的 通过使用实时光谱反馈信息来评估手术结果。 大部分工作将在贝克曼激光研究所完成。 加州尔湾大学，设备齐全，支持体内 准分子激光角膜手术和视力的临床研究 研究计划。贝克曼激光研究所位于相当接近 该部门的办公室是密切隶属于该部的 眼科贝克曼激光研究所内有一台临床准分子激光器， 本研究将使用的激光（VISX Star 2）。此外，贝克曼 激光研究所包含一个设备齐全的饲养兔子的动物园， 将用于这些研究的实验动物 和超微结构设备也可在研究所内使用。 UCI眼科有活动性角膜和外部疾病 的服务.该临床实践将为患者提供临床 在这项研究的最后阶段。

项目成果

A technique to harvest Descemet's membrane with viable endothelial cells for selective transplantation.

• DOI: 10.1016/j.ajo.2004.09.068
• 发表时间: 2005-02
• 期刊: American journal of ophthalmology
• 影响因子: 4.2
• 作者: Teresa S. Ignacio;Thao T B Nguyen;M. Sarayba;P. Sweet;O. Piovanetti;R. Chuck;A. Behrens

High expression of macrophage migration inhibitory factor in the murine lacrimal gland.

小鼠泪腺中巨噬细胞迁移抑制因子的高表达。

• DOI: 10.1097/ico.0b013e3181c06466
• 发表时间: 2010
• 期刊: Cornea
• 影响因子: 2.8
• 作者: Park,ChoulYong;Shin,YoungJoo;Lekhanont,Kaevalin;Zhang,Cheng;Lee,Woo-Seok;Cano,Marisol;Bucala,Richard;Chuck,RoyS
• 通讯作者: Chuck,RoyS

Endothelial lamellar keratoplasty using an artificial anterior chamber and a microkeratome.

使用人工前房和微型角膜刀进行内皮板层角膜移植术。

• DOI: 10.1001/archopht.121.4.503
• 发表时间: 2003
• 期刊: Archives of ophthalmology (Chicago, Ill. : 1960)
• 作者: Behrens,Ashley;Ellis,Kenneth;Li,Li;Sweet,PaulaM;Chuck,RoyS
• 通讯作者: Chuck,RoyS