TRANSCRIPTIONAL REGULATION OF THE FSH RECEPTOR

描述：(改编自申请人的摘要)这项工作的目的 应用于分析FSHR基因启动子的顺式作用 相互作用的元件，最终是转录因子 该基因的激素反应性和支持细胞特异性表达。 目的1：FSHR基因近端启动子的扫描突变 将被用来识别对表达很重要的DNA反应元件 支持细胞系及支持细胞的原代培养。在目标2中， 促卵泡激素、表皮生长因子和转化生长因子-β对FSHR基因表达的影响 学习。首先，经原发灶处理后的FSHR的编码基因 我们将分析含有FSH、EGF或TGFbeta的Sertoli细胞培养。 第二，FSHR启动子对这些基因的转录反应 物质将通过Sertoli的瞬时表达分析来解决 细胞。第三，FSHR启动子与FSHR共表达 参与促卵泡激素、表皮生长因子、 或进行转化生长因子β信号转导。

DESCRIPTION: (adapted from the applicant's abstract) The objective of this application is to analyze the promoter for the FSHR gene for cis-acting elements and, ultimately, transcription factors that interact to mediate hormonal responsiveness and Sertoli cell-specific expression of this gene. In aim 1, scanning mutagenesis of the proximal promoter of the FSHR gene will be used to identify DNA response elements important for expression in a Sertoli cell line and primary cultures of Sertoli cells. In aim 2, the effects of FSH, EGF, and TGF-beta on the expression of the FSHR gene will be studied. First, the mRNA encoding the FSHR following treatment of primary cultures of Sertoli cells with either FSH, EGF, or TGFbeta will be analyzed. Second, the transcriptional response of the FSHR promoter to these same substances will be addressed by transient expression assays in Sertoli cells. Third, co-expression of FSHR promoter constructs with constitutively active forms of intracellular kinases that are involved in either FSH, EGF, or TGFbeta signal transduction will be performed.