乳头瘤病毒 DNA 复制——宿主细胞因素-猫眼课题宝

乳头瘤病毒 DNA 复制——宿主细胞因素

批准号：

6386744

负责人：

THOMAS MELENDY

金额：

$11.51万

依托单位：

STATE UNIVERSITY OF NEW YORK AT BUFFALO

依托单位国家：

美国

项目类别：

财政年份：

1997

资助国家：

美国

项目状态：

已结题

起止时间：

1997-08-01 至 2003-07-31

来源：

https://reporter.nih.gov/project-details/6386744

关键词：

DNA binding protein DNA directed DNA polymerase DNA replication Papillomavirus helicase host organism interaction molecular cloning protein kinase protein purification virus DNA virus infection mechanism

中文摘要

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英文摘要

DESCRIPTION: Papillomaviruses are important human pathogens whose biology is still incompletely understood. This grant is focused on unraveling the repertoire of proteins required for papillomavirus DNA replication in vitro. Reconstituting viral DNA synthesis in vitro has been used before as an invaluable means of understanding the host's replication machinery. Such experiments with SV40 have provided tremendous insight into the basic DNA replication apparatus within mammalian cells. The repertoire of purified proteins sufficient to direct SV40 DNA snthesis in vitro is not sufficient to direct papillomavirus DNA synthesis in vitro. The major goal of this grant is to isolate and characterize the remaining celllular activities required for papillomavirus DNA replication.

期刊论文(14)

专著(0)

科研奖励(0)

会议论文

Bizelesin, a bifunctional cyclopropylpyrroloindole alkylating agent, inhibits simian virus 40 replication in trans by induction of an inhibitor.

Bizelesin 是一种双功能环丙基吡咯并吲哚烷化剂，通过抑制剂的诱导抑制猿病毒 40 反式复制。

DOI： 10.1021/bi990598r

发表时间： 1999

期刊：

Biochemistry

影响因子： 2.9

作者：

[McHugh,MM, Kuo,SR, Walsh-O'Beirne,MH, Liu,JS, Melendy,T, Beerman,TA]

通讯作者： Beerman,TA

DNA damage by the enediyne C-1027 results in the inhibition of DNA replication by loss of replication protein A function and activation of DNA-dependent protein kinase.

烯二炔 C-1027 造成的 DNA 损伤会导致复制蛋白 A 功能丧失和 DNA 依赖性蛋白激酶激活，从而抑制 DNA 复制。

DOI： 10.1021/bi015680c

发表时间： 2001

期刊：

Biochemistry

影响因子： 2.9

作者：

[Liu,JS, Kuo,SR, Yin,X, Beerman,TA, Melendy,T]

通讯作者： Melendy,T

The cellular response to DNA damage induced by the enediynes C-1027 and neocarzinostatin includes hyperphosphorylation and increased nuclear retention of replication protein a (RPA) and trans inhibition of DNA replication.

由烯二炔 C-1027 和新制癌菌素诱导的 DNA 损伤的细胞反应包括过度磷酸化和复制蛋白 a (RPA) 的核保留增加以及 DNA 复制的反式抑制。

DOI： 10.1021/bi001668t

发表时间： 2001

期刊：

Biochemistry

影响因子： 2.9

作者：

[McHugh,MM, Yin,X, Kuo,SR, Liu,JS, Melendy,T, Beerman,TA]

通讯作者： Beerman,TA

The Rep protein of adeno-associated virus type 2 interacts with single-stranded DNA-binding proteins that enhance viral replication.

2 型腺相关病毒的 Rep 蛋白与单链 DNA 结合蛋白相互作用，增强病毒复制。

DOI： 10.1128/jvi.78.1.441-453.2004

发表时间： 2004

期刊：

Journal of virology

影响因子： 5.4

作者：

[Stracker,TravisH, Cassell,GeoffreyD, Ward,Peter, Loo,Yueh-Ming, vanBreukelen,Bas, Carrington-Lawrence,StacyD, Hamatake,RobertK, vanderVliet,PeterC, Weller,SandraK, Melendy,Thomas, Weitzman,MatthewD]

通讯作者： Weitzman,MatthewD

Mcm2, but not RPA, is a component of the mammalian early G1-phase prereplication complex.

DOI： 10.1083/jcb.146.4.709

发表时间： 1999-08-23

期刊：

The Journal of cell biology

影响因子： --

作者：

[Dimitrova DS, Todorov IT, Melendy T, Gilbert DM]

通讯作者： Gilbert DM