CORE--TISSUE PROCUREMENT AND ANALYSIS

组织获取和分析核心设施提供了一个完整的 不仅提供组织获取、处理和 向SPORE调查人员发放组织， 重要的分析组件。核心是扩展这个分析 考虑到成本， 有效地集中资源，并由专家维护 质量控制和质量保证做法。采购科 有效地进入所有乳房外科手术， 所有患者的样本和可用的新鲜组织， 同意.将组织输入数据库并分发给 根据要求，孢子研究者可以作为处理过的DNA、RNA或组织切片。 外周血淋巴细胞的树皮也保持在所有乳房 患者组织分析部分提供免疫组织化学和 其他蛋白质分析的孢子。本节的一个主要重点是 提供了对新基因的分析，这些基因是通过 基础科学项目，评估这些基因的表达水平， 在正常和恶性乳腺组织中。这一部分正在扩大， 还包括SPORE的基于人群的研究（项目#2： 卡罗莱纳乳腺癌研究，项目3：DCIS研究，项目5：马约 良性乳腺疾病研究）的肿瘤抑制基因的表达， 癌基因和增殖标记物。蛋白质产物的表达 这些基因将与分子分析相关， 获得的暴露信息。

The tissue procurement and analysis core facility serves an integral function of not only providing tissue procurement, processing and disbursement of tissue to SPORE investigators but also provides an important analysis component. The core is expanding this analysis component to the population based studies of the SPORE to allow for cost effective centralization of resources coupled with expert maintenance of quality control and quality assurance practices. The procurement section efficiently accesses all breast surgical procedures and obtains blood samples and when available fresh tissue on all patients giving informed consent. The tissues are entered in a database and distributed to the SPORE investigators as processed DNA, RNA or tissue sections as requested. A bark of peripheral blood lymphocytes is also maintained on all breast patients. The tissue analysis section provides immunohistochemical and other protein analysis for the SPORE. A major focus of this section provides analyses for the novel genes which have been discovered via the basic science projects, assessing the levels of expression of these genes in normal and malignant breast tissues. This section is being expanded to also include the population based studies of the SPORE (Project#2: Carolina Breast Cancer Study, Project#3: DCIS Study, Project#5: Mayo Benign Breast Disease Study) for the expression of tumor suppressor genes, oncogenes and proliferation markers. Expression of the protein products of these genes will be related to the molecular analyses as well as to exposure information obtained.