CORE--TISSUE PROCUREMENT AND ANALYSIS

组织采购和分析核心设施是 功能不仅提供纸巾的采购、加工和 组织支付给孢子调查人员，但也提供了一种 重要的分析组件。核心是扩展这一分析 孢子种群研究的组成部分，以考虑成本 资源的有效集中以及专家的维护 质量控制和质量保证实践。采购科 高效地访问所有乳房手术程序并获取血液 所有患者的样本和新鲜组织在可用时给予通知 同意。这些组织被输入数据库并分发给 孢子调查员按要求处理DNA、RNA或组织切片。 所有乳房上也有外周血淋巴细胞的树皮。 病人。组织分析部分提供免疫组织化学和 对孢子进行其他蛋白质分析。这一部分的一个主要焦点 提供了对通过 基础科学项目，评估这些基因的表达水平 在正常和恶性乳腺组织中均有表达。这一部分将扩展到 还包括以种群为基础的孢子研究(项目2： 卡罗莱纳乳腺癌研究，项目3：DCIS研究，项目5：Mayo 良性乳腺疾病研究)用于肿瘤抑制基因的表达， 癌基因和增殖标记物。猪瘟病毒蛋白产物的表达 这些基因将与分子分析有关，也与 获取的曝光信息。

The tissue procurement and analysis core facility serves an integral function of not only providing tissue procurement, processing and disbursement of tissue to SPORE investigators but also provides an important analysis component. The core is expanding this analysis component to the population based studies of the SPORE to allow for cost effective centralization of resources coupled with expert maintenance of quality control and quality assurance practices. The procurement section efficiently accesses all breast surgical procedures and obtains blood samples and when available fresh tissue on all patients giving informed consent. The tissues are entered in a database and distributed to the SPORE investigators as processed DNA, RNA or tissue sections as requested. A bark of peripheral blood lymphocytes is also maintained on all breast patients. The tissue analysis section provides immunohistochemical and other protein analysis for the SPORE. A major focus of this section provides analyses for the novel genes which have been discovered via the basic science projects, assessing the levels of expression of these genes in normal and malignant breast tissues. This section is being expanded to also include the population based studies of the SPORE (Project#2: Carolina Breast Cancer Study, Project#3: DCIS Study, Project#5: Mayo Benign Breast Disease Study) for the expression of tumor suppressor genes, oncogenes and proliferation markers. Expression of the protein products of these genes will be related to the molecular analyses as well as to exposure information obtained.