TRANSDUCTION AND RETINAL DEGENERATION IN DROSOPHILA

果蝇的转导和视网膜变性

• 批准号: 6329556
• 负责人: PATRICK J. DOLPH
• 金额: $ 17.61万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 2002-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6329556
• 关键词: Drosophilidae; SDS polyacrylamide gel electrophoresis; alleles; arrestins; electrophysiology; gene induction /repression; gene mutation; genetic mapping; immunocytochemistry; laboratory rabbit; molecular cloning; nucleic acid sequence; phosphorylation; protein structure function; retina degeneration; rhodopsin; visual phototransduction

DESCRIPTION (Adapted from applicant's abstract): While not identical to vertebrate systems, Drosophila phototransduction is similar in several respects, and the power of the genetic system justifies its use as a model. Extensive genetic screens have been conducted by this applicant for genes that can affect the rate of retinal degeneration which follows from altered rhodopsin:arrestin ratios. In all, 350,000 F1 flies screened have yielded 112 new dominant mutations. This innovative approach stands in contrast to the huge effort required to discover phototransduction mutants directly by their ERG phenotypes. There are four specific aims. In the first aim, the applicant will study the ten new arrestin alleles in the collection by determining the null ERG phenotype, and examining the role of phosphorylation, rhodopsin binding and the function of the arrestin C-terminal domain. The second aim focuses on the ten new rhodopsin mutants that cause retinal degeneration, some of which do so in a novel light-dependent manner. The third aim involves the molecular isolation and characterization of a new retinal degeneration locus recovered four times in the screen. The fourth aim is to pursue the characterization of the other mutations recovered in the screens.

描述（改编自申请人摘要）：虽然不完全相同

项目成果

Epitope masking of rhabdomeric rhodopsin during endocytosis-induced retinal degeneration.

• 发表时间: 2002-12
• 期刊: Molecular vision
• 影响因子: 2.2
• 作者: N. Orem;P. Dolph

Loss of the phospholipase C gene product induces massive endocytosis of rhodopsin and arrestin in Drosophila photoreceptors.

磷脂酶 C 基因产物的缺失会诱导果蝇感光体中视紫质和视紫红质的大量内吞作用。

• DOI: 10.1016/s0042-6989(01)00229-2
• 发表时间: 2002
• 期刊: Vision research
• 影响因子: 1.8
• 作者: Orem,NicholasR;Dolph,PatrickJ
• 通讯作者: Dolph,PatrickJ