TRANSDUCTION AND RETINAL DEGENERATION IN DROSOPHILA

果蝇的转导和视网膜变性

• 批准号: 6125133
• 负责人: PATRICK J. DOLPH
• 金额: $ 17.09万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6125133
• 关键词: Drosophilidae; SDS polyacrylamide gel electrophoresis; alleles; arrestins; electrophysiology; gene induction /repression; gene mutation; genetic mapping; immunocytochemistry; laboratory rabbit; molecular cloning; nucleic acid sequence; phosphorylation; protein structure function; retina degeneration; rhodopsin; visual phototransduction

DESCRIPTION (Adapted from applicant's abstract): While not identical to vertebrate systems, Drosophila phototransduction is similar in several respects, and the power of the genetic system justifies its use as a model. Extensive genetic screens have been conducted by this applicant for genes that can affect the rate of retinal degeneration which follows from altered rhodopsin:arrestin ratios. In all, 350,000 F1 flies screened have yielded 112 new dominant mutations. This innovative approach stands in contrast to the huge effort required to discover phototransduction mutants directly by their ERG phenotypes. There are four specific aims. In the first aim, the applicant will study the ten new arrestin alleles in the collection by determining the null ERG phenotype, and examining the role of phosphorylation, rhodopsin binding and the function of the arrestin C-terminal domain. The second aim focuses on the ten new rhodopsin mutants that cause retinal degeneration, some of which do so in a novel light-dependent manner. The third aim involves the molecular isolation and characterization of a new retinal degeneration locus recovered four times in the screen. The fourth aim is to pursue the characterization of the other mutations recovered in the screens.

描述（根据申请人的摘要改编）：虽然与 脊椎动物系统，果蝇的光转导相似 尊重，遗传系统的力量证明其用作模型。 该申请人已经为基因进行了广泛的遗传筛选 这可能会影响因改变而随之而来的视网膜变性率 Rhodopsin：逮捕蛋白比。 总共筛选了350,000 F1苍蝇 112新的主要突变。 这种创新的方法与 直接发现光转传突变体所需的巨大努力 他们的ERG表型。 有四个具体目标。 在第一个目标中， 申请人将研究集合中的十个新逮捕等位基因 确定零ERG表型，并检查 磷酸化，视紫红质结合和抑制素的功能 C末端域。 第二个目的重点是十个新的视紫红质突变体 这会导致视网膜变性，其中一些在小说中这样做 光依赖性方式。 第三个目标涉及分子隔离和 新的视网膜变性基因座的表征恢复了四次 屏幕。 第四个目标是追求对方的特征 在屏幕中恢复的突变。