KINASE MEDIATED SIGNALING IN THE RICE DEFENSE RESPONSE

水稻防御反应中激酶介导的信号传导

• 批准号: 6342948
• 负责人: PAMELA C RONALD
• 金额: $ 17.84万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6342948
• 关键词: Pseudomonadaceae; biological signal transduction; gene expression; phosphorylation; plant diseases; plant genetics; plant proteins; protein kinase; rice; tissue /cell culture; yeast two hybrid system

Receptor kinases (RKs) play an important role in the mechanisms witch which organisms perceive and respond to environmental signals; regulating growth and development in both animals and plants. The long-term goal of the proposed project is to elicit the signal transaction cascade regulated by RKs. This information will enhance our understanding of cellular transformation and carcinogenesis in animals and lead to the development of environmentally safe strategies for disease control in plants. The short-term goal of the proposed project is to establish the causal connection between pathogen induced activation of the rice receptor-like kinase, XA21, and the downstream defense responses. Based on information from receptor tyrosine kinases in animal systems, we hypothesize that the catalytic domain of XA21 plays a key role in XA21-mediated signal transduction and that specific residues as binding sites for intracellular proteins. These proteins may then directly or indirectly transduce various defense responses. We also hypothesize that the pathogen may secrete factors into the host cell that influence the signal transduction pathway. In partial support of these hypotheses, we have found that the intracellular domain can autophosphorylate itself in vitro, that a pathogen GTP-binding protein and a rice catalase interact specifically with the XA21 in plants undergoing a resistance response. To test these hypotheses, we propose to carry out the following objectives: 1. Confirm the essential role of phosphorylation in XA21-mediated disease resistance. 2. Identify and characterize rice proteins that interact with the Xa21- kinase. 3. Characterize the defense response and defense-related gene expression in the XA21-mediated defense pathway. 4. Determine the biological significance of the xoo/GBP interaction. Accomplishments of the proposed objectives will, for the first time, lead to the establishment of the causal connection between a plant receptor kinase and the associated downstream response.

受体激酶（RKS）在机制中起重要作用 哪些生物会感知并响应环境信号；调节 动物和植物的生长和发育。长期目标 拟议的项目是引起受监管的信号交易级联 由RKS。这些信息将增强我们对蜂窝的理解 动物的转化和致癌作用，并导致发展 植物中疾病控制的环境安全策略。 拟议项目的短期目标是建立因果关系 病原体引起的水稻受体激活之间的联系 激酶，XA21和下游防御反应。基于信息 从动物系统中的受体酪氨酸激酶中，我们假设 XA21的催化结构域在XA21介导的信号中起关键作用 转导和特定残基作为细胞内的结合位点 蛋白质。这些蛋白质可能会直接或间接转导各种 国防反应。我们还假设病原体可以分泌 影响宿主细胞的因素，影响信号转导途径。 为了部分支持这些假设，我们发现 细胞内结构域可以在体外自动磷酸化，即 病原体GTP结合蛋白和水稻过氧化氢酶特异性相互作用 植物中的XA21经历了抗性反应。测试这些 假设，我们建议执行以下目标：1。 磷酸化在XA21介导的疾病抗性中的基本作用。 2。识别和表征与XA21-相互作用的水稻蛋白 激酶。 3。表征国防反应和与防御有关的基因 在XA21介导的防御途径中的表达。 4。确定 XOO/GBP相互作用的生物学意义。成就 拟议的目标将首次导致建立 植物受体激酶与相关的因果关系 下游响应。