Subthreshold ion currents in the rat locomotor CPG

大鼠运动 CPG 中的亚阈值离子电流

• 批准号: 6404993
• 负责人: Ronald M Harris-Warrick
• 金额: $ 4.94万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6404993
• 关键词: axon; cell type; chordate locomotion; gene expression; genetic mapping; interneurons; laboratory rat; membrane potentials; neural transmission; neurophysiology; polymerase chain reaction; postdoctoral investigator; serotonin; spinal cord; voltage /patch clamp

Understanding the spinal circuits that organize rhythmic limb movements is critical for future treatment of patients with spinal cord injuries. The overall goal of this project is to further our understanding of a set of intemeurons that participate in the central pattern generator (CPG) for locomotion in the neonatal rat spinal cord. The commissural intemeurons (CINs) send their axons to the contralateral spinal cord and are thought to play critical roles in left-right coordination and rhythm generation during locomotion. I propose to characterize two of the ionic currents that control post-inhibitory rebound in three anatomically defined classes of CINs with ascending, descending or intrasegmental axons. Using whole cell voltage clamp recordings, I will measure the parameters of the hyperpolarization-activated inward current, Ih and the low-threshold calcium current, IT, in each cell type, and determine the effects of serotonin on these currents. I will perform single cell RT- PCR studies to determine which genes related to Ih and IT are expressed in each CIN class. These experiments should provide further criteria for rigorous identification of neuronal classes in the CPG for locomotion in the neonatal rat spinal cord.

了解组织有节奏的肢体运动的脊髓回路对于脊髓损伤患者的未来治疗至关重要。该项目的总体目标是进一步了解一组中间神经元，这些中间神经元参与新生大鼠脊髓运动的中央模式发生器（CPG）。连合中间神经元（CIN）将轴突发送到对侧脊髓，被认为在运动过程中的左右协调和节律产生中发挥着关键作用。我建议描述在具有上升、下降或节段内轴突的三种解剖学定义类别的 CIN 中控制抑制后反弹的两种离子电流。使用全细胞电压钳记录，我将测量每种细胞类型中超极化激活的内向电流 Ih 和低阈值钙电流 IT 的参数，并确定血清素对这些电流的影响。我将进行单细胞 RT-PCR 研究，以确定与 Ih 和 IT 相关的哪些基因在每个 CIN 类别中表达。这些实验应该为严格识别 CPG 中用于新生大鼠脊髓运动的神经元类别提供进一步的标准。