PHYSIOLOGICAL ROLES OF ION CHANNELS IN A SMALL NETWORK

小网络中离子通道的生理作用

• 批准号: 6195865
• 负责人: Ronald M Harris-Warrick
• 金额: $ 39.17万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-09 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6195865
• 关键词: Malacostraca; Xenopus; Xenopus oocyte; calcium channel; double stranded RNA; electrophysiology; gene induction /repression; neurogenetics; neurons; polymerase chain reaction; potassium channel; pyloric region; voltage gated channel

DESCRIPTION: (Adapted from the Investigator's Abstract) Simple behaviors are generated by networks of neurons, interacting in complex and nonlinear ways. The output from a behavioral network is determined by the pattern and strength of synaptic interactions within the network as well as the unique intrinsic firing properties of the different network neurons. These synaptic and firing properties are generated in turn by the differing ratios of ion channels and receptor genes that are expressed by each neuron in the network. Our goal is to bridge the span from gene expression to neural network function, to show how single genes contribute to shaping the behavioral output from a neural network. To accomplish this, we are studying the roles of a set of potassium and calcium channel genes within the 14-neuron pyloric network in the lobster stomatogastric ganglion. This network is one of the best-described neural networks: all of the synaptic connections are known and the firing properties of all the neurons are well understood. We propose to manipulate gene expression for ion channels in single identified neurons in this network to better understand the rules of network function. There are 3 specific aims to accomplish this goal. First, we will complete our ongoing project to clone the genes for and analyze the biophysical properties of the Shaker family of voltage dependent K+ channels as well as two calcium channels. Second, we will determine which neurons in the pyloric network express these genes, and where in the neuron the proteins are targeted. Third, we will increase the expression of these genes in single neurons, using injections of sense RNA, or decrease expression with injections of dominant negative, antisense or double-stranded RNA, and determine how this alters the firing and synaptic properties of the neuron and the pattern of activity in the pyloric network. This "molecular neuroethological" approach will allow us to study the effects of one gene and thus one channel at a time on the behavioral output of a network, which in the past has only been possible with theoretical mathematical models.

描述：（根据调查员的摘要进行了改编） 简单的行为是由神经元网络产生的，在复杂的 和非线性方式。行为网络的输出由 网络内突触相互作用的模式和强度以及 不同网络神经元的独特内在触发特性。这些 突触和发射特性又通过不同的比率而产生 由每个神经元表达的离子通道和受体基因 网络。我们的目标是弥合从基因表达到神经网络的跨度 功能，显示单个基因如何促进行为输出 来自神经网络。为此，我们正在研究集合的角色 14-神经元幽门网络中钾和钙通道基因的 龙虾气孔神经节。该网络是描述最好的网络之一 神经网络：所有突触连接都是已知的，并且发射 所有神经元的特性都被充分理解。我们建议操纵 该网络中单个鉴定神经元中离子通道的基因表达 更好地了解网络功能的规则。有3个特定目标 实现这一目标。首先，我们将完成我们正在进行的克隆的项目 用于振动家族的生物物理特性的基因 依赖电压的K+通道以及两个钙通道。第二，我们会的 确定幽门网络中的哪些神经元表达这些基因，以及哪些神经元 在神经元中，蛋白质是靶向的。第三，我们将增加表达 这些基因在单个神经元中使用感官RNA或减少 注射主要负，反义或双链的表达 RNA，并确定这如何改变点火和突触特性 神经元和幽门网络中的活动模式。这个“分子 神经媒性的方法将使我们能够研究一个基因的影响和 因此，一次在网络的行为输出上一次通道 过去只有理论数学模型才有可能。