Development of a system for simultaneous multiplane image acquisition for live cell

开发活细胞同时多平面图像采集系统

• 批准号: 1666608
• 金额: --
• 依托单位: University of Kent
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1666608
• 关键词: Development; system; simultaneous; multiplane; image

During this project the student will develop a system to allow truly synchronous multi-plane image acquisition, which will significantly improve the time-resolution during 3d-live-cell imaging. Cairn have achieved successful simultaneous imaging at two different depths into a sample. The student will develop and extend this technique using Cairn's novel Multisplit device, which allows an image to be split into 4 equivalent images on the same camera chip. The student will integrate lenses into this apparatus to obtain different focal lengths to image 4 discrete z-planes on the chip. They will then develop this method and use a combination of piezo and light path splitters to optimize control and stability of spatial distribution between images to permit z distances to be defined by the user, according to experimental needs. The aims for the student are to:1. Develop a simultaneous 4-plane image acquisition system with the aim of making the method widely available.2. Make a detailed high temporal resolution multi-dimension study of rapid movements of myosins and dynamic rearrangements of the actin cytoskeleton to further our understanding of their regulation and function

在这个项目中，学生将开发一个系统，允许真正同步的多平面图像采集，这将显着提高三维活细胞成像的时间分辨率。Cairn已经成功地在样品的两个不同深度同时成像。学生将使用Cairn的新型Multissplit设备开发和扩展这种技术，该设备允许将图像分割为同一相机芯片上的4个等效图像。学生将把透镜集成到这个装置中，以获得不同的焦距，在芯片上成像4个离散的z平面。然后，他们将开发这种方法，并使用压电和光路分离器的组合来优化图像之间空间分布的控制和稳定性，以允许用户根据实验需求定义z距离。学生的目标是：1。研制了一套四平面同步图像采集系统，旨在使该方法得到广泛应用.对肌球蛋白的快速运动和肌动蛋白骨架的动态重排进行详细的高时间分辨率的多维研究，以进一步了解其调控和功能

项目成果

Temperature sensitive point mutations in fission yeast tropomyosin have long range effects on the stability and function of the actin-tropomyosin copolymer.

裂变酵母菌菌素中温度敏感的点突变对肌动蛋白 - 肌球蛋白共聚物的稳定性和功能具有长度的影响。

• DOI: 10.1016/j.bbrc.2017.10.109
• 发表时间: 2018-11-25
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Johnson CA;Brooker HR;Gyamfi I;O'Brien J;Ashley B;Brazier JE;Dean A;Embling J;Grimsey E;Tomlinson AC;Wilson EG;Geeves MA;Mulvihill DP
• 通讯作者: Mulvihill DP

A novel live-cell imaging system reveals a reversible hydrostatic pressure impact on cell-cycle progression.

• DOI: 10.1242/jcs.212167
• 发表时间: 2018-08-06
• 期刊: Journal of cell science
• 影响因子: 4
• 作者: Brooker HR;Gyamfi IA;Wieckowska A;Brooks NJ;Mulvihill DP;Geeves MA
• 通讯作者: Geeves MA