Real-time cell metabolism by NMR

通过 NMR 实时细胞代谢

• 批准号: 1734216
• 金额: --
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1734216
• 关键词: Real; time; cell; metabolism; NMR

We have recently published the first study of real-time metabolomics carried out by Nuclear Magnetic Resonance (NMR) of living primary cancer cells from patients. Real-time metabolomics observes a time course of metabolism in cells. In this project a flow cell developed by Bruker will be adapted for real-time studies in cells, whereby we will study the differentiation of progenitorcells to myeloid cells in the presence or absence of a specific transcription factor. Applying a novel NMR approach will open avenues for exciting new metabolic analyses in cell lines and in patient derived cells. The PhD candidate will be part of a leading national NMR centre, will be involved in the development of a probe for real-time NMR in cells with Bruker and will learn to prepare cell samples for metabolic analysis

我们最近发表了第一项通过核磁共振（NMR）对患者的活原代癌细胞进行实时代谢组学研究。实时代谢组学观察细胞中代谢的时间过程。在该项目中，布鲁克公司开发的流动细胞将适用于细胞中的实时研究，从而我们将研究在存在或不存在特定转录因子的情况下祖细胞向骨髓细胞的分化。应用新的NMR方法将为细胞系和患者衍生细胞中令人兴奋的新代谢分析开辟途径。博士候选人将成为领先的国家核磁共振中心的一员，将参与布鲁克公司开发细胞实时核磁共振探针，并将学习制备用于代谢分析的细胞样品。