MECHANISMS OF CELLULAR TAURINE TRANSPORT IN BRAIN EDEMA

脑水肿中细胞牛磺酸转运机制

基本信息

批准号：
6393925
负责人：
JAMES E OLSON
金额：
$ 25.33万
依托单位：
WRIGHT STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-04-15 至 2004-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6393925
关键词：
animal tissue astrocytes biological signal transduction biological transport brain edema cell morphology cell type electrophysiology hippocampus homeostasis immunocytochemistry neurons pathologic process taurine tissue /cell culture tissue /cell preparation

项目摘要

DESCRIPTION (Adapted from applicant's abstract): Cytotoxic brain edema is a serious complication of several clinical conditions including stroke, liver failure and traumatic brain injury and is characterized by swelling of astrocytes while volumes of neurons and other cells are relatively unaffected. It has been established that cell volume regulation in isolated astrocytes and neurons depends to some extent on the synthesis and transport of taurine. The central hypothesis of the application is that taurine is transported from neurons to astroglial cells during the development of cytotoxic brain edema, a process of regulation of neuronal volume. Based upon this hypothesis, it is proposed: (1) to determine swelling-induced changes in cell volume and taurine content of neurons and glia in hippocampal slices; (2) to test the proposal that taurine efflux from neurons is more sensitive and is distinct from that from astrocytes; (3) to characterize signal transduction pathways involved in taurine efflux in neurons versus astrocytes; (4) to examine volume regulation, electrophysiology and ion homeostasis in hippocampal slices exposed to osmotic edema. It is suggested that these studies will provide a better understanding of mechanisms of brain cell volume regulation under physiological conditions and the response of brain cells to pathological swelling, the knowledge of which will help in understanding cytotoxic brain edema in clinical states and in developing novel therapies.

描述（根据申请人的摘要改编）：细胞毒性脑水肿是一种多种临床状况的严重并发症，包括中风，肝脏衰竭和脑部损伤，其特征是肿胀星形胶质细胞虽然神经元和其他细胞的体积相对不受影响。已经确定，细胞体积调节孤立的星形胶质细胞和神经元在一定程度上取决于牛磺酸的合成和运输。这该应用的中心假设是牛磺酸是从神经元在细胞毒性脑水肿的发展过程中向星形胶质细胞（A）神经元体积调节的过程。基于这个假设，是提议：（1）确定细胞体积和牛磺酸的肿胀诱导的变化海马切片中神经元和神经胶质的含量；（2）测试提案神经元的牛磺酸流出更敏感，与此不同来自星形胶质细胞；（3）表征涉及的信号转导途径神经元与星形胶质细胞中的牛磺酸外排；（4）检查体积调节，暴露于渗透的海马切片中的电生理学和离子稳态浮肿。建议这些研究将提供更好的理解生理条件下脑细胞体积调节机制脑细胞对病理肿胀的反应，了解这将有助于理解临床状态和在开发新型疗法中。

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Calcium/calmodulin-modulated chloride and taurine conductances in cultured rat astrocytes.

培养的大鼠星形胶质细胞中钙/钙调蛋白调节氯离子和牛磺酸电导。

DOI：
10.1016/s0006-8993(01)03235-8
发表时间：
2002
期刊：
Brain research
影响因子：
2.9
作者：
Li,Guangze;Liu,Yin;Olson,JamesE
通讯作者：
Olson,JamesE

Asymmetrical response of p38 kinase activation to volume changes in primary rat astrocytes.

p38 激酶激活对原代大鼠星形胶质细胞体积变化的不对称反应。

DOI：
10.1177/153537020122601008
发表时间：
2001
期刊：
Experimental biology and medicine (Maywood, N.J.)
影响因子：
0
作者：
Xu,D;Wang,L;Olson,JE;Lu,L
通讯作者：
Lu,L

Taurine and cellular volume regulation in the hippocampus.

牛磺酸和海马细胞体积调节。

DOI：
10.1007/978-1-4615-0077-3_14
发表时间：
2003
期刊：
Advances in experimental medicine and biology
影响因子：
0
作者：
Olson,JamesE;Kreisman,NormanR;Lim,Jenny;Hoffman-Kuczynski,Beth;Schelble,Dana;Leasure,James
通讯作者：
Leasure,James

Osmotic sensitivity of taurine release from hippocampal neuronal and glial cells.

海马神经元和神经胶质细胞释放牛磺酸的渗透敏感性。