Different Notch Receptors in Drosophila Development

果蝇发育中的不同Notch受体

• 批准号: 6455379
• 负责人: CEDRIC S WESLEY
• 金额: $ 22.65万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6455379
• 关键词: Drosophilidae; arthropod genetics; biological signal transduction; cell differentiation; developmental genetics; developmental neurobiology; protein binding; receptor expression

DESCRIPTION (provided by applicant): Notch receptors generate different types of differentiation signals during development of humans. Accordingly, their loss or aberrant activities result in different types of developmental defects and diseases. To understand the basis for these maladies, and be able to deftly deal with each one of them, we must understand all the different signals generated by these receptors. Drosophila offers an ideal system for the purpose. During differentiation of the central nervous system and epidermis in Drosophila embryos, Notch generates four to five different types of signals. The signals generated to specify the epidermal precursor cells are well understood. However, very little is known about the other types of Notch signals, the intracellular pathways transducing them, or the mechanism by which they are generated. Recent studies show that the full-length Notch receptor, the primary receptor, generates two secondary receptors showing different activities than the primary receptor. The different activities of the three receptors together have the potential to account for all the different types of differentiation signals generated by Notch. The proposed studies will use molecular genetic and biochemical analyses to determine the following. (1) The nature of in vivo signals generated by the different Notch receptors at different stages of differentiation. (2) Whether or not signals from the secondary receptors are transduced by the same pathway that transduces the epidermal precursor cell signals. (3) Whether proteins known to bind Notch regulate the production and activities of the different receptors. These studies are expected to lead to an understanding of how the primary and secondary Notch receptors regulate differentiation of tissues during development.

描述（由申请人提供）：Notch 受体产生不同类型 人类发育过程中的分化信号。因此，他们的 丧失或异常活动会导致不同类型的发育缺陷 和疾病。了解这些疾病的根源，并能够熟练地治疗 处理每一个信号，我们必须理解所有不同的信号 由这些受体产生。果蝇提供了一个理想的系统 目的。在中枢神经系统和表皮分化过程中 在果蝇胚胎中，Notch 会产生四到五种不同类型的信号。 产生的指定表皮前体细胞的信号很好 明白了。然而，人们对其他类型的 Notch 知之甚少。 信号、转导信号的细胞内途径或机制 它们是生成的。最近的研究表明，全长Notch受体， 主要受体，产生两个显示不同的次级受体 活性高于主要受体。三者的活动不同 受体一起有可能解释所有不同类型的 Notch 产生的分化信号。拟议的研究将使用 分子遗传和生化分析可确定以下内容。 (1) 的 不同Notch受体产生的体内信号的性质 分化的不同阶段。 (2) 信号是否来自 次级受体的转导途径与转导的途径相同 表皮前体细胞信号。 (3) 是否已知结合Notch的蛋白质 调节不同受体的产生和活性。这些 预计研究将有助于了解主要和 次级Notch受体在过程中调节组织的分化 发展。