权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Different Notch Receptors in Drosophila Development

果蝇发育中的不同Notch受体

基本信息

批准号：
7100000
负责人：
CEDRIC S WESLEY
金额：
$ 3万
依托单位：
UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-07-01 至 2006-06-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Notch receptors generate different types of differentiation signals during development of humans. Accordingly, their loss or aberrant activities result in different types of developmental defects and diseases. To understand the basis for these maladies, and be able to deftly deal with each one of them, we must understand all the different signals generated by these receptors. Drosophila offers an ideal system for the purpose. During differentiation of the central nervous system and epidermis in Drosophila embryos, Notch generates four to five different types of signals. The signals generated to specify the epidermal precursor cells are well understood. However, very little is known about the other types of Notch signals, the intracellular pathways transducing them, or the mechanism by which they are generated. Recent studies show that the full-length Notch receptor, the primary receptor, generates two secondary receptors showing different activities than the primary receptor. The different activities of the three receptors together have the potential to account for all the different types of differentiation signals generated by Notch. The proposed studies will use molecular genetic and biochemical analyses to determine the following. (1) The nature of in vivo signals generated by the different Notch receptors at different stages of differentiation. (2) Whether or not signals from the secondary receptors are transduced by the same pathway that transduces the epidermal precursor cell signals. (3) Whether proteins known to bind Notch regulate the production and activities of the different receptors. These studies are expected to lead to an understanding of how the primary and secondary Notch receptors regulate differentiation of tissues during development.

描述(申请人提供)：缺口受体产生不同的类型人类发育过程中的分化信号。因此，他们的丧失或异常活动会导致不同类型的发育缺陷和疾病。了解这些疾病的基础，并能够熟练地处理它们中的每一个，我们必须理解所有不同的信号由这些受体产生。果蝇提供了一种理想的系统目的。在中枢神经系统和表皮分化过程中在果蝇胚胎中，Notch会产生四到五种不同类型的信号。产生的用于指定表皮前体细胞的信号很好明白了。然而，人们对其他类型的切槽知之甚少信号，细胞内传递信号的途径，或通过什么机制它们是生成的。最近的研究表明，全长Notch受体，初级受体产生两个次级受体，表现出不同的比初级受体更有活性。三者的不同活动受体一起有可能解释所有不同类型的由Notch产生的差动信号。拟议的研究将使用进行分子遗传和生化分析，以确定以下各项。(1) 不同Notch受体在体内产生的信号的性质分化的不同阶段。(2)信号是否来自次级受体的转导途径与转导表皮前体细胞发出信号。(3)已知与Notch结合的蛋白质调节不同受体的产生和活动。这些预计研究将导致理解小学和小学是如何次级Notch受体调节组织分化发展。