CREATING A DNA MICROARRAY AND CDNA SPOTTING FACILITY

创建 DNA 微阵列和 CDNA 点样设施

• 批准号: 6200109
• 负责人: DAFNA BAR-SAGI
• 金额: $ 24.62万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2002-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6200109
• 关键词: 3T3 cells; biomedical facility; complementary DNA; fibroblast growth factor; gene induction /repression; growth factor receptors; hormone regulation /control mechanism; interferons; interleukin 4; microarray technology; multiple myeloma; neoplasm /cancer genetics; prolactin; protooncogene

DESCRIPTION: (Applicant's Description) The School of Medicine proposes to establish a DNA microarray and cDNA spotting facility to enhance the established programs of National Cancer lnstitute funded and newly-developing cancer researchers at Stony Brook. The application seeks support largely towards the acquisition of a turn-key DNA microarray system and a separate scanner to support an existing cDNA spotting capability. Strong institutional support is provided towards a successful application, approximately 2.5-fo1d over the support provided by an award. The institution has a proven track record in creating and maintaining core facilities and will assume the responsibility to establish and to manage a core facility in DNA microarray and cDNA spotting. The established programs funded by the National Cancer Institute include: expression profiling of genes that are regulated by Ras and are relevant to oncogenesis; characterization of transcriptional activation/deactivation induced by two members of the vav family of proto- oncogenes; analysis of the expression of specific genes encoding molecules associated with surface proteases in carcinoma progression; microarraying to characterize the role of critical cell cycle regulated transcription factors in yeast; analysis of transcriptional targets of the Runt domain proteins; analysis of gene expression regulated by FGFR3 in leukemia multiple myeloma cell lines; identification of genes regulated by expression of E2f complexes containing viral or cellular regulatory proteins; identification of genes regulated by the expression of the various isoforms of the p73 gene; and profiling of the genes regulated by the growth regulatory hormones interferon, prolactin, and interleukin-4. Experts in computational problems of microarraying will work in concert with the cancer biologists to address bioinformatic issues central to the optimal use of these technologies. The Core Advisory Board will be created (chaired by the applicant) and populated by users to provide oversight and to manage issues of technical operation, access, and upgrade of the facility in the future, as needed.

描述：（申请人的描述）医学院建议， 建立DNA微阵列和cDNA点样设备，以提高 国家癌症研究所资助和新开发的项目 斯托尼布鲁克的癌症研究人员。该申请主要寻求支持 对收购交钥匙DNA微阵列系统和一个单独的 扫描仪，以支持现有的cDNA点样能力。坚强体制 为成功申请提供支持，约2.5-fo 1d 一个奖项所提供的支持。该机构有一个行之有效的轨道 创造和维护核心设施的记录，并将承担 负责建立和管理DNA微阵列的核心设施 和cDNA点样。由国家癌症基金会资助的既定项目 研究所包括：由Ras调控的基因的表达谱， 与肿瘤发生有关;转录特征 激活/失活诱导的两个成员的vav家族的原， 癌基因.特定基因编码分子表达的分析 与癌进展中的表面蛋白酶相关;微阵列， 表征关键细胞周期调节转录因子的作用 分析Runt结构域蛋白的转录靶点; 白血病多发性骨髓瘤中FGFR 3调控基因表达分析 细胞系; E2 f复合物表达调节基因的鉴定 含有病毒或细胞调节蛋白;基因鉴定 受p73基因的各种同种型的表达调节;和 由生长调节激素干扰素调节的基因的谱分析， 催乳素和白细胞介素-4。计算问题的专家 微阵列将与癌症生物学家合作， 生物信息学问题是优化利用这些技术的核心。的 将创建核心咨询委员会（由申请人担任主席），并由其成员组成 由用户提供监督和管理技术操作问题， 今后视需要对设施进行升级。