MECHANISMS OF EUKARYOTIC TRANSCRIPTIONAL REGULATION

描述（取自应用程序） 申请者请求对Keystone会议提供部分支助， “真核转录调控机制”将于2月举行。 2001年3月4日，新墨西哥州圣达菲。在这个时候召开会议的目的 是将顶尖科学家和有前途的年轻研究人员聚集在一起， 在迅速发展的，越来越多的 转录调控的多学科领域。基因控制 表达是一个涉及染色质调节的多步骤过程 促进RNA聚合酶和转录相互作用的结构 与DNA模板的因子以及聚合酶的直接调节， 转录的起始前、起始和延伸阶段。 最近，高分辨率晶体学和核磁共振结构的 核小体、RNA聚合酶和调节转录的复合物 已经确定了活化、延伸和组蛋白乙酰化。的 这些结构研究的信息，以及正在进行的生物化学研究， 转录起始和延伸的分析，提供了一个 更清晰地描绘了转录下的分子相互作用 控制此外，蛋白质的发现和随后的表征 复合物和酶活性作为染色质重塑 转录因子的机器、辅激活子、辅阻遏子或介质 正在解开许多复杂的监管机制， 高等生物体内的生理信号重要的新信息是 还涉及染色体水平上的基因控制， 核定位的变化。此外，全基因组分析提供了 新的见解转录调控电路在细胞和 有机体水平。总的来说，在这次会议上提出的信息 与我们对异常控制机制的理解直接相关， 是许多人类疾病的基础这次会议的重点是审查每一个 主要水平的转录调控的细节，并制定一个连贯的 了解基因表达的特异性是如何实现的， 染色体和生理背景。参加本次会议的人员应 对研究生的职业生涯特别重要， 希望专攻这一研究领域的博士后研究员。

DESCRIPTION (taken from the application) The applicant requests partial support for the Keystone Conference on "Mechanisms of Eukaryotic Transcriptional Regulation" to be held during Feb. 26-Mar. 4, 2001 in Santa Fe, NM. The purpose of holding a meeting at this time is to bring together leading scientists and promising young investigators to present their latest findings in the rapidly moving and increasingly multidisciplinary field of transcriptional regulation. The control of gene expression is a multi-step process that involves modulation of chromatin structure to facilitate the interaction of RNA polymerase and transcription factors with the DNA template as well as direct regulation of polymerase during the preinitiation, initiation, and elongation stages of transcription. Recently, high-resolution crystallographic and NMR structures of the nucleosome, RNA polymerase, and complexes that regulate transcriptional activation, elongation, and histone acetylation have been determined. The information from these structural studies, together with ongoing biochemical analyses of transcriptional initiation and elongation, are providing an ever-clearer picture of the molecular interactions underlying transcriptional control. Additionally, the discovery and subsequent characterization of protein complexes and enzymatic activities that function as chromatin remodeling machines, co-activators, co-repressors, or mediators of transcription factors are unraveling many of the sophisticated regulatory mechanisms that integrate physiological signals within higher organisms. Important new information is also being generated concerning gene control at the chromosomal level and by changes in nuclear localization. Moreover, whole genome analysis is providing new insight into transcriptional regulatory circuitry at the cellular and organismal levels. Taken together, the information presented at this conference has direct relevance to our understanding of aberrant control mechanisms that underlie many human diseases. The focus of this meeting is to examine each major level of transcriptional regulation in detail and develop a coherent understanding of how specificity of gene expression is achieved in a chromosomal and a physiological context. Participation at this meeting should be of particular importance to the careers of graduate students and postdoctoral fellows hoping to specialize in this area of research.