MECHANISMS OF EUKARYOTIC TRANSCRIPTIONAL REGULATION

描述(取自应用程序) 申请人请求部分支持#年的Keystone会议 “真核转录调控机制”将于2月2日举行。 3月26日-3月4,2001年，新墨西哥州圣达菲。在这个时候召开会议的目的 是把顶尖的科学家和有前途的年轻研究人员聚集在一起 将他们的最新发现呈现在日新月异的 转录调控的多学科领域。基因调控 表达是一个多步骤的过程，涉及染色质的调节 促进RNA聚合酶与转录相互作用的结构 DNA模板的影响因素以及聚合酶的直接调节 转录的起始前、起始和延伸阶段。 最近，高分辨率的结晶学和核磁共振结构 核小体、RNA聚合酶和调节转录的复合体 活性、延伸率和组蛋白乙酰化已被测定。这个 来自这些结构研究的信息，以及正在进行的生化 对转录起始和延伸的分析，提供了一种 转录基础上的分子相互作用的图景越来越清晰 控制力。此外，蛋白质的发现和随后的特性 起染色质重塑作用的复合体和酶活性 转录因子的机器、共激活因子、共抑制因子或介体 正在瓦解许多复杂的监管机制，这些机制将 高等生物体内的生理信号。重要的新信息是 也是关于染色体水平上的基因控制和通过 核局部化的变化。此外，全基因组分析提供了 对细胞和细胞中转录调控电路的新见解 生物体水平。综上所述，在这次会议上提出的信息 与我们对异常控制机制的理解有直接关系 这是许多人类疾病的根源。这次会议的重点是审查每一个 主要水平的转录调控的详细和发展的连贯 了解基因表达的特异性是如何在 染色体和生理背景。参加这次会议应该 对研究生的职业生涯特别重要， 希望专门从事这一领域研究的博士后研究员。

DESCRIPTION (taken from the application) The applicant requests partial support for the Keystone Conference on "Mechanisms of Eukaryotic Transcriptional Regulation" to be held during Feb. 26-Mar. 4, 2001 in Santa Fe, NM. The purpose of holding a meeting at this time is to bring together leading scientists and promising young investigators to present their latest findings in the rapidly moving and increasingly multidisciplinary field of transcriptional regulation. The control of gene expression is a multi-step process that involves modulation of chromatin structure to facilitate the interaction of RNA polymerase and transcription factors with the DNA template as well as direct regulation of polymerase during the preinitiation, initiation, and elongation stages of transcription. Recently, high-resolution crystallographic and NMR structures of the nucleosome, RNA polymerase, and complexes that regulate transcriptional activation, elongation, and histone acetylation have been determined. The information from these structural studies, together with ongoing biochemical analyses of transcriptional initiation and elongation, are providing an ever-clearer picture of the molecular interactions underlying transcriptional control. Additionally, the discovery and subsequent characterization of protein complexes and enzymatic activities that function as chromatin remodeling machines, co-activators, co-repressors, or mediators of transcription factors are unraveling many of the sophisticated regulatory mechanisms that integrate physiological signals within higher organisms. Important new information is also being generated concerning gene control at the chromosomal level and by changes in nuclear localization. Moreover, whole genome analysis is providing new insight into transcriptional regulatory circuitry at the cellular and organismal levels. Taken together, the information presented at this conference has direct relevance to our understanding of aberrant control mechanisms that underlie many human diseases. The focus of this meeting is to examine each major level of transcriptional regulation in detail and develop a coherent understanding of how specificity of gene expression is achieved in a chromosomal and a physiological context. Participation at this meeting should be of particular importance to the careers of graduate students and postdoctoral fellows hoping to specialize in this area of research.