CONFOCAL MICROSCOPE

共焦显微镜

• 批准号: 6051611
• 负责人: DIANA L KUNZE
• 金额: $ 19.11万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6051611
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

This grant requests funds to upgrade a Noran Odyssey XL confocal microscope presently part of the shared facilities in the Rammelkamp Research Institute. This machine was purchased shortly after its introduction into the market. The capacity for image acquisition and analysis has been enhanced since the original purchase of the Odyssey XL. The upgrade (Noran OZ) will provide important and needed additional laser and software with deconvolution power and, finally, higher spatial and time resolution. Training, management and accounting procedures are already in pace. The use of the present machine by the major investigators listed below has been in general two areas. One major use has been to identify and localized with high resolution the intracellular and extracellular proteins in a variety of tissues using immunocytochemical agents, fluorescent track tracers and GFP as signals. Tissue include cultured cell lines expressing particular ion channel constructs (HEK cells, L cells), cultured cells (fibroblasts, endothelial sf9 cells), primary cultures (neuronal, myocytes, epithelial, parathyroid), and tissue slices (brain, heart, kidney). The second major use has been dyes such as calcium, membrane potential and other indicators to assess the roles of these ions in cellular function. In this method we take advantage of the time resolution as will as spatial resolution of the technique. These measurements are combined with electrophysiological measurements. They have been used for studying localized calcium influx in endothelial and other cells in response to agonists and studies of voltage-sensitive dye changes in arterial baroreceptor terminals.

该赠款要求资金升级Rammelkamp Research Institute目前共享设施的一部分的Noran Odyssey XL共聚焦显微镜。这台机器是在推出市场后不久购买的。自从原始购买Odyssey XL以来，图像获取和分析的能力已得到增强。升级（Noran Oz）将提供具有反卷积功率的重要且需要的其他激光和软件，最后，更高的空间和时间分辨率。培训，管理和会计程序已经处于步伐状态。以下列出的主要研究人员使用现有机器的使用通常是两个领域。一种主要用途是使用免疫细胞化学剂，荧光轨迹示踪剂和GFP作为信号识别和本地化的细胞内和细胞外蛋白质。组织包括表达特定离子通道构建体（HEK细胞，L细胞），培养细胞（成纤维细胞，内皮SF9细胞），原发性培养物（神经元，肌细胞，上皮，甲状旁腺）和组织切片（大脑，心脏，肾脏）的培养细胞系。第二个主要用途是染料，例如钙，膜电位和其他指标，以评估这些离子在细胞功能中的作用。在这种方法中，我们将利用时间分辨率，就像该技术的空间分辨率一样。这些测量与电生理测量相结合。它们已用于研究激动剂的内皮和其他细胞中局部钙的涌入以及对动脉压力感受器末端的电压敏感染料变化的研究。