Mechanism of Homeostatic Plasticity in a Visceral Sensory Circuit

内脏感觉回路的稳态可塑性机制

• 批准号: 7352617
• 负责人: DIANA L KUNZE
• 金额: $ 40.06万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7352617
• 关键词: Accounting; Address; Animals; Back; Brain; Calcium; Calcium Channel; Cardiovascular system; Carotid Body; Chemoreceptors; Chronic; Data; Development; Electric Stimulation; Fiber; Frequencies; Gene Expression; Genetic Techniques; Goals; Heart Rate; Homeostasis; Hypertension; Hypoxia; Intervention; Label; Membrane Proteins; Modeling; Molecular Genetics; Monitor; Neural Pathways; Neuraxis; Neurons; Nucleus solitarius; Output; Pathway interactions; Pattern; Physiological; Presynaptic Terminals; Principal Investigator; Procedures; Progress Reports; Protein Analysis; Proteins; Reflex action; Sensory; Site; Sleep Apnea Syndromes; Slice; Stimulus; Synapses; Synaptic Membranes; Synaptic Transmission; Telemetry; Time; Visceral; design; falls; insight; neurotransmission; neurotransmitter release; pressure; presynaptic; programs; protein distribution; relating to nervous system; research study; respiratory; respiratory reflex; response; transmission process; voltage

In response to changing afferent input, neural pathways may undergo both short term (minutes) and long term (days, weeks) adjustments within the pathway in order to maintain physiological output within an appropriate range. The underlying cellular mechanisms responsible for this homeostatic adaptation may include changes in gene expression and subsequent protein distribution/function. In this study we examine the mechanisms that underlie the long term changes in the central neural component of a cardio-respiratory reflex response to chronic intermittent hypoxia (CIH), a model of sleep apnea. We are using a combination of electrophysiological, molecular and genetic techniques to understand the adaptations (over days/weeks) to CI H that produce sustained changes in chemosensory synaptic transmission in the nucleus of the solitary tract leading to an elevated level of information transfer that is partially restrained by what we propose is a secondary homeostatic adaptation. These changes are thought to contribute to elevated arterial pressure and exaggerated chemoreflexes in CIH. In the first of two years we focus on the mechanisms underlying an increase in spontaneous release of neurotransmitter that occurs after three days of CIH. In the second year we address mechanisms underlying the adaptive response to this exaggerated response and that brings synaptic transmission partially back towards normal. We believe this study is important not only because it provides insight to potential mechanisms for manipulating respiratory and cardiovascular reflexes at specific sites but also because it provides insight to adaptations in neuronal transmission that may be universal in application.

为了响应变化的传入输入，神经途径可能会经历短期（分钟）和长时间 途径内的术语（天，几周）调整，以维持生理产量 适当的范围。负责这种稳态适应的基本细胞机制可能 包括基因表达的变化和随后的蛋白质分布/功能。在这项研究中，我们检查了 长期变化的心脏呼吸器中心神经组成部分的机制是 对慢性间歇性缺氧（CIH）的反射反应，这是睡眠呼吸暂停模型。我们正在使用组合 电生理，分子和遗传技术以了解适应性（几天/几周） 到在孤立的核中产生化学感应突触传播持续变化的CI H 导致信息传输水平升高，这部分受我们建议的是 次级体内稳态适应。这些变化被认为会导致动脉压升高 并在CIH中夸大化学反射。在两年的头两年中，我们专注于一个机制 CIH三天后发生的神经递质的自发释放增加。第二年 我们解决了对这种夸张反应的适应性反应的基础机制，并带来 突触传播部分返回正常。我们认为这项研究很重要，不仅是因为 为特定的呼吸道和心血管反射操纵的潜在机制提供了见解 网站也是因为它可以洞悉适应神经元传播的，这可能是普遍的 应用。