INTEGRATED UPGRADE OF THE NMR INSTRUMENTATION FACILITY

普林斯顿大学化学系向国家研究资源中心(NCRR)申请资金，用于核磁共振仪器设备的综合升级，重点是生物分子和生物分析应用。这次集成升级的核心是一台新的400 MHz核磁共振光谱仪，它将取代过时的机器，并消除常规和半常规应用的瓶颈。同时，这一改进将使现有的高场光谱仪更有效地用于要求苛刻的生物分子研究。综合升级的其他项目将扩大这些高场仪器的能力。它们将开辟新的研究机会，满足关键的研究需求，主要是在生物分子的结构、表征和动力学研究领域。这些单元是：一个完整的LC-核磁共振配件套件，一个慢速旋转的高分辨率MAS探针头，一个13C-15N检测优化的三重共振探针头，以及一台用于高效离线数据分析和结构计算的现代计算机。这一升级对系内研究项目的成功至关重要，特别是对由11名教员组成的核心用户群体的研究项目。实施后，核磁共振仪器设施的集成升级将极大地扩展我们的总能力，并以适度的总投资提高对生物结构研究至关重要的最高场核磁共振光谱仪和相关装置的效率。

The Department of Chemistry at Princeton University requests funds from the National Center for Research Resources (NCRR) for the purpose of an integrated upgrade of the NMR instrumentation facility, with the focus on biomolecular and bioanalytical applications. The centerpiece for this integrated upgrade is a new 400 MHz NMR spectrometer, which will replace an outdated machine and remove the bottleneck of routine and semi-routine applications. At the same time this enhancement will allow more efficient use of the existing high-field spectrometers for demanding biomolecular research. Additional items of the integrated upgrade will extend the capacity of these high-field instruments. They will open new research opportunities and serve critical research needs primarily in the field of structure, characterization, and dynamics studies of biomolecules. These units are: a complete LC-NMR accessory kit, a slow-spinning high- resolution MAS probehead, a 13C-15N detection optimized triple resonance probehead and a modern computer for efficient off-line data analysis and structure calculations. This upgrade is critical to the success of the research projects in the Department, specifically those of the core user group of eleven faculty members. When implemented, this integrated upgrade of the NMR instrumentation facility will have greatly expanded our total capabilities and increased the efficiency of the highest field NMR spectrometers and associated units, critical for biostructural studies , for a modest total investment.