600 MHZ NUCLEAR MAGNETIC RESONANCE SPECTROMETER

600 MHz 核磁共振波谱仪

• 批准号: 6052090
• 负责人: ANDREW P HINCK
• 金额: $ 40万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-14 至 2001-04-13
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6052090
• 关键词: biomedical equipment purchase; intermolecular interaction; nuclear magnetic resonance spectroscopy; structural biology

A high field NMR spectrometer is needed to support ongoing studies of biomolecular structure at the University of Texas Health Science Center at San Antonio. If funded, the proposed spectrometer will be dedicated to biological-oriented research that requires state-of-the-art high-field NMR instrumentation and relatively long, continuous blocks of instrument time. The proposed instrument will greatly enhance the research of seven major users. Common features of this diverse group of users include: 1) An immediate need for on site high-field biomolecular NMR instrumentation, 2) Research problems dedicated towards solving fundamental problems in contemporary biochemistry, and 3) NIH or NSF supported research programs, having a significant NMR component. Research in the following diverse areas will be significantly advanced by the proposed instrument: (1) Cytokine-cytokine research and Protein- RNA interactions (Hinck), (2) The effects of amino acid substitutions on the structure, stability, and folding pathways of cytochrome c (Nall), (3) The role of PDZ domains in mediating protein localization (Masters), (4) Flexibility and function in the apical domain of the chaperonin GroEL (Horowitz), (5) Substrate specificities and mechanism of the mutT family of phosphohydrolases (Barnes), (6) Cytochrome exidase-cytochrome c interactions as probed by H-D functional labeling (Robinson), and (7) Core histone tail domain interactions (Hansen). The request is justified by the urgent need for high field NMR instrumentation in San Antonio with the ability to perform triple resonance and pulsed field gradient experiments that are essential to the data collection needs of our user base. The proposed instrument has the strong endorsement of the University. The requested funds of $400,000 will be combined with $454,000 from sources within the University of Texas Health Sciences Center at San Antonio. The University will also provide the resources to cover instrument installation, maintenance, and the salary for the NMR facility manager. The instrument will become a core component of the Institutions Center for Biomolecular Structure Analysis, a comprehensive center for macromolecular structure determination, which occupies over 11,000 ft2 of newly completed research space in the Allied Health Sciences Building on the University of Texas Health Science Center at San Antonio campus. The instrument will operate as a shared resource serving the University of Texas Health Science Center at San Antonio and the South Texas research community.

圣安东尼奥的德克萨斯大学健康科学中心需要一台高场核磁共振光谱仪来支持正在进行的生物分子结构研究。如果获得资助，拟议的光谱仪将致力于生物导向的研究，需要最先进的高场核磁共振仪器和相对较长的，连续的仪器时间块。拟议的工具将大大加强七个主要用户的研究。 这些不同用户群的共同特征包括：1）现场高场生物分子NMR仪器的迫切需求，2）致力于解决当代生物化学基本问题的研究问题，以及3）NIH或NSF支持的研究计划，具有重要的NMR组成部分。拟议的文书将大大推进以下不同领域的研究：（1）细胞因子-细胞因子研究和蛋白质- RNA相互作用（Hinck），（2）氨基酸取代对细胞色素c的结构、稳定性和折叠途径的影响（Nall），（3）PDZ结构域在介导蛋白质定位中的作用（Masters），（4）伴侣蛋白GroEL的顶端结构域中的柔性和功能（Horowitz），（5）磷酸水解酶mutT家族的底物特异性和机制（巴恩斯），（6）通过H-D功能标记探测的细胞色素氧化酶-细胞色素c相互作用（罗宾逊），和（7）核心组蛋白尾部结构域相互作用（汉森）。这一要求是合理的，迫切需要高场核磁共振仪器在圣安东尼奥的能力，进行三重共振和脉冲场梯度实验，是必不可少的数据收集需求，我们的用户群。拟议的文书得到联合国大学的大力支持。所要求的40万美元资金将与来自圣安东尼奥德克萨斯大学健康科学中心的45.4万美元资金相结合。该大学还将提供资源，以支付仪器安装，维护和核磁共振设施经理的工资。该仪器将成为生物分子结构分析机构中心的核心组成部分，该中心是一个综合性的大分子结构测定中心，位于德克萨斯大学健康科学中心圣安东尼奥校区的联合健康科学大楼内，占地超过11，000平方英尺。该仪器将作为一个共享资源，为位于圣安东尼奥的得克萨斯大学健康科学中心和南得克萨斯研究社区服务。

项目成果

Characterization of hydride transfer to flavin adenine dinucleotide in neuronal nitric oxide synthase reductase domain: geometric relationship between the nicotinamide and isoalloxazine rings.

神经元一氧化氮合酶还原酶结构域中氢化物转移至黄素腺嘌呤二核苷酸的表征：烟酰胺和异咯嗪环之间的几何关系。

• DOI: 10.1006/abbi.2001.2588
• 发表时间: 2001
• 期刊: Archives of biochemistry and biophysics.
• 作者: Miller,RT;Hinck,AP
• 通讯作者: Hinck,AP

Backbone resonance assignments of the 48 kDa dimeric putative 18S rRNA-methyltransferase Nep1 from Methanocaldococcus jannaschii.

来自詹纳斯基甲烷球菌的 48 kDa 二聚体推定 18S rRNA-甲基转移酶 Nep1 的主链共振分配。

• DOI: 10.1007/s12104-009-9187-z
• 发表时间: 2009
• 期刊: Biomolecular NMR assignments
• 影响因子: 0.9
• 作者: Wurm,JanPhilip;Duchardt,Elke;Meyer,Britta;Leal,BelindaZ;Kötter,Peter;Entian,Karl-Dieter;Wöhnert,Jens
• 通讯作者: Wöhnert,Jens