600 MHZ NUCLEAR MAGNETIC RESONANCE SPECTROMETER

圣安东尼奥的德克萨斯大学健康科学中心需要一台高场核磁共振光谱仪来支持正在进行的生物分子结构研究。如果获得资金，建议的光谱仪将致力于以生物为导向的研究，这需要最先进的高场核磁共振仪器和相对较长、连续的仪器时间块。拟议的仪器将极大地加强对七个主要用户的研究。这一多样化的用户群体的共同特征包括：1)对现场高场生物分子核磁共振仪器的迫切需求，2)致力于解决当代生物化学中的基本问题的研究问题，以及3)NIH或NSF支持的具有重要核磁共振成分的研究计划。该仪器将显著推进以下不同领域的研究：(1)细胞因子-细胞因子研究和蛋白质-RNA相互作用(Hinck)，(2)氨基酸取代对细胞色素c(NALL)结构、稳定性和折叠途径的影响，(3)PDZ结构域在介导蛋白质定位(Master)中的作用，(4)伴侣蛋白GroEL(Horowitz)顶端区域的灵活性和功能，(5)磷酸水解酶MUT家族(Barnes)的底物特异性和机制，(6)H-D功能标记(Robinson)检测的细胞色素氧化酶-细胞色素c相互作用；(7)核心组蛋白尾部结构域相互作用(Hansen)。圣安东尼奥迫切需要高场核磁共振仪器，能够进行三重共振和脉冲场梯度实验，这对我们用户的数据收集需求至关重要。拟议的文书得到了该大学的大力支持。申请的40万美元资金将与圣安东尼奥德克萨斯大学健康科学中心来源的454,000美元结合在一起。该大学还将提供资源，用于支付仪器安装、维护和核磁共振设施经理的工资。该仪器将成为机构生物分子结构分析中心的核心组件，该中心是一个综合的大分子结构确定中心，位于德克萨斯大学圣安东尼奥校区健康科学中心的联合健康科学大楼内，新建成的研究空间超过11,000平方英尺。该仪器将作为共享资源，为位于圣安东尼奥的德克萨斯大学健康科学中心和南得克萨斯州研究社区服务。

A high field NMR spectrometer is needed to support ongoing studies of biomolecular structure at the University of Texas Health Science Center at San Antonio. If funded, the proposed spectrometer will be dedicated to biological-oriented research that requires state-of-the-art high-field NMR instrumentation and relatively long, continuous blocks of instrument time. The proposed instrument will greatly enhance the research of seven major users. Common features of this diverse group of users include: 1) An immediate need for on site high-field biomolecular NMR instrumentation, 2) Research problems dedicated towards solving fundamental problems in contemporary biochemistry, and 3) NIH or NSF supported research programs, having a significant NMR component. Research in the following diverse areas will be significantly advanced by the proposed instrument: (1) Cytokine-cytokine research and Protein- RNA interactions (Hinck), (2) The effects of amino acid substitutions on the structure, stability, and folding pathways of cytochrome c (Nall), (3) The role of PDZ domains in mediating protein localization (Masters), (4) Flexibility and function in the apical domain of the chaperonin GroEL (Horowitz), (5) Substrate specificities and mechanism of the mutT family of phosphohydrolases (Barnes), (6) Cytochrome exidase-cytochrome c interactions as probed by H-D functional labeling (Robinson), and (7) Core histone tail domain interactions (Hansen). The request is justified by the urgent need for high field NMR instrumentation in San Antonio with the ability to perform triple resonance and pulsed field gradient experiments that are essential to the data collection needs of our user base. The proposed instrument has the strong endorsement of the University. The requested funds of $400,000 will be combined with $454,000 from sources within the University of Texas Health Sciences Center at San Antonio. The University will also provide the resources to cover instrument installation, maintenance, and the salary for the NMR facility manager. The instrument will become a core component of the Institutions Center for Biomolecular Structure Analysis, a comprehensive center for macromolecular structure determination, which occupies over 11,000 ft2 of newly completed research space in the Allied Health Sciences Building on the University of Texas Health Science Center at San Antonio campus. The instrument will operate as a shared resource serving the University of Texas Health Science Center at San Antonio and the South Texas research community.