PHILIPS XL30 SCANNING ELECTRON MICROSCOPE

飞利浦 XL30 扫描电子显微镜

• 批准号: 6052084
• 负责人: ROBERT S MCCUSKEY
• 金额: $ 13.86万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6052084
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; scanning electron microscopy

There are severe limitations for conventional scanning electron microscopy of biological material. These include limitations on resolution, lack of back-scattered detection, and the inability to image uncoated, non-conductive samples at low accelerating voltages. All of these capabilities are critically needed by a number of NIH funded investigators and lack of access to them at the University of Arizona is seriously limiting our productivity and progress. As a result, we are requesting funds to purchase a Philips XL30 SEM equipped with a conventional secondary electron detector for optimal detection under high vacuum conditions, and a backscattered electron detector. The Philips XL30 SEM was selected as the instrument on the market having the features which make it the most suitable instrument for the needs of our major user group. These needs include the ability to: (a) obtain high resolution secondary electron images at low voltage on the surfaces of fixed, critical-point dried, but uncoated sample, (c) obtain backscattered electron images of uncoated samples to reveal internal structure as well as the distribution heavy metal (e.g., colloidal gold) labeling of surface structures; (d) record these images in photographic video, or digital format for subsequent presentation, archiving, and/or image analysis. The research plans demonstrate our immediate and critical need for an SEM not only to replace our now defunct, 22 year-old conventional SEM at the College of Medicine Imaging Faculty, but also to provide high-resolution conventional SEM capabilities to eight NIH-funded major users, and at least six NIH-funded minor users in a variety of departments within the University of Arizona and one investigator from the University of Southern California who collaborates with the Principal Investigator for the electron microscopy required for her project.

传统的扫描电子显微镜对生物材料的研究存在严重的局限性。这些包括分辨率的限制，缺乏背散射检测，以及无法在低加速电压下对未涂覆的非导电样品进行成像。所有这些能力都是一些NIH资助的研究人员迫切需要的，亚利桑那大学缺乏这些能力严重限制了我们的生产力和进步。因此，我们正在申请资金购买一台飞利浦XL 30扫描电镜，该扫描电镜配备了传统的二次电子检测器，可在高真空条件下进行最佳检测，还配备了背散射电子检测器。Philips XL 30 SEM被选为市场上具有使其成为最适合我们主要用户群体需求的仪器的功能的仪器。这些需要包括以下能力：（a）在固定的、临界点干燥的但未涂覆的样品的表面上在低电压下获得高分辨率二次电子图像，（c）获得未涂覆的样品的背散射电子图像以揭示内部结构以及重金属（例如，胶体金）标记表面结构;（d）以摄影视频或数字格式记录这些图像，用于随后的呈现、存档和/或图像分析。研究计划表明，我们迫切需要一个扫描电镜，不仅要取代我们现在已经不复存在的，22岁的传统扫描电镜在医学成像学院的学院，而且要提供高分辨率的传统扫描电镜的能力，以八个NIH资助的主要用户，至少有六个国家卫生研究院资助亚利桑那大学各部门的未成年人用户和一名来自南加州大学的研究人员，他们与她的项目所需的电子显微镜的主要研究者。