LASER SCANNING CONFOCAL MICROSCOPE

这是一个建议，要求NCRR资金购买激光扫描共聚焦显微镜系统，以支持多学科资助的研究在凯斯西储大学。 来自9个系的17名受资助的教师被确定为主要的初始用户。 该仪器将被建立为NCI指定的综合癌症中心的共享资源。 癌症中心将提供5万美元用于购买该系统，并将支持服务合同费用和设施经理工资的50%。 除了启动支持外，癌症中心还致力于通过NCI资助的癌症中心支持赠款提供长期支持。显微镜系统包括具有扫描头的Zeiss LSM 510成像系统、提供458和488 nm线的氩激光器、提供543和633 nm线的HeNe激光器、UV激光器、4个PMT检测器、振动隔离台和具有DIC和高分辨率油和水浸没光学器件的Axiovert 100 M倒置荧光显微镜。 它将用于检查已用荧光探针标记的单个活细胞和组织切片。 新的共聚焦系统将由凯斯西储大学癌症中心的成员使用，他们没有足够的机会使用共聚焦显微镜。

This is a proposal to request NCRR funds for purchase of a laser scanning confocal microscope system to support multi-disciplinary funded research at Case Western Reserve University. A group of 17 funded faculty from nine departments are identified as major initial users. The instrument will be established as a shared resource in the NCI-designated Comprehensive Cancer Center. The Cancer Center will provide 50,000 dollars toward the purchase of the system and will support service contract costs and 50 percent of the facility manager's salary. In addition to start-up support, the Cancer Center is committed to long term support through the NCI-funded Cancer Center Support Grant. The microscope system consists of a Zeiss LSM 510 imaging system with a scanning head, an argon laser providing 458 and 488 nm lines, HeNe lasers providing 543 and 633 nm lines, a UV laser, 4 PMT detectors, a vibration isolation table, and an Axiovert 100M inverted fluorescence microscope with DIC and high resolution oil and water immersion optics. It will be used for examination of single living cells and tissue slices that have been labeled with fluorescent probes. The new confocal system will be used by members of the Cancer Center who do not have adequate access to confocal microscopy at Case Western Reserve University.