CONFOCAL MICROSCOPY CORE

共焦核心设施的目标是提供最先进的仪器， 单个活细胞和组织的亚微米三维分辨率。蔡司LSM 510 激光扫描共聚焦显微镜配备多个激光器，提供多种激发谱线。一 可调谐脉冲ThSapphire激光器（700-900 nm）提供200飞秒脉冲，用于两个和三个光子 激发单光子激发截面在紫外线内的各种荧光团， 可见光光谱倒置Nikon Eclipse TE 2000-S荧光显微镜，由 MetaMorph允许采集宽场荧光、亮场和相衬图像。该设施 服务于癌症中心的成员，以及更广泛的凯斯西储大学社区， 每周7天开放。仪器上的时间仅通过预订，癌症中心成员 总是优先于非会员。设施主任全面负责操作 为核心用户提供活细胞共聚焦和多光子方面的专业知识和咨询 显像设施经理对设施的显微镜部分负有日常责任 在主任的监督下。咨询委员会每年举行会议，以解决与 设施运营、新技术开发和政策制定。咨询委员会向 癌症中心高级领导委员会核心是从事研究生教学， 组织一个2学分的研究生课程，光学显微镜和成像的生物学家。学生获得 在经验丰富的专家指导下，使用最先进的光学成像设备进行广泛的实践培训 学术导师。总的来说，共聚焦显微镜核心将对以下方面产生巨大而积极的影响： 癌症中心的科学项目此外，不同部门之间使用该设施， 中心将促进个体研究者之间富有成效的互动，以促进新的合作。

The objective of the Confocal Core Facility is to provide state-of-the-art instrumentation for examination of single living cells and tissues with submicron 3-dimensional resolution. The facility houses a Zeiss LSM 510 laser scanning confocal microscope equipped with multiple lasers to provide a variety of excitation lines. A tunable pulsed ThSapphire laser (700-900 nm) provides 200 femtosecond pulses for two and three-photon excitation of a variety of fluorophores whose one-photon excitation cross sections are in the ultra violet and visible light spectrum. An inverted Nikon Eclipse TE2000-S fluorescence microscope controlled by MetaMorph allows acquisition of wide-field fluorescence, bright field, and phase contrast images. The facility serves members of the Cancer Center, as well as the broader Case Western Reserve University community, and is open 7 days per week. Time on the instrument is by reservation only, and Cancer Center members will always have priority over nonmembers. The facility director has overall responsibility for the operations of the facility and provides expertise and consultation to core users in live cell confocal and multiphoton imaging. The facility manager has day-to-day responsibility for the microscopy component of the facility under the supervision of the director. An advisory committee meets annually to address any issues with facility operations, new technology development, and setting of policy. The advisory committee reports to the Senior Leaders Committee of the Cancer Center. The core is engaged in graduate teaching by organizing a 2-credit graduate course, Optical Microscopy and Imaging for Biologists. Students gain extensive hands-on training with state-of-the-art equipment for optical imaging guided by experienced academic instructors. Collectively, the Confocal Microscopy Core will have a large and positive impact on the Cancer Center's scientific programs. In addition, the use of the facility among different departments and centers will foster productive interactions between individual investigators to promote new collaborations.