PROCISE 491 CLC PROTEIN SEQUENCER

这是一项NIH-NCRR共享仪器拨款，用于为印第安纳大学医学院购买ABI Procise 491 cLC蛋白测序仪。我们有四个由美国国立卫生研究院资助的核心用户组，他们将占据90%的可用仪器时间。这些项目的标题是：1)确定蛋白激酶磷酸化靶点的基因组方法；2)鉴定负责调节支链α -酮酸脱氢酶和丙酮酸脱氢酶复合物的磷酸蛋白磷酸酶；3)鉴定和结构表征钙摄取和释放所需的心脏肌浆蛋白；4)鉴定肝星状细胞中类视黄醇代谢酶。另外还有10个nih资助的核心用户表示对蛋白质测序感兴趣，他们将占用剩余的10%的仪器时间。在医学院校园里没有普遍可用的蛋白质测序仪。Procise cLC将被放置在印第安纳大学医学院新成立的跨学科结构生物学中心，该中心的目标是为对生物大分子结构特性表征感兴趣的研究人员提供最先进的仪器和教师顾问。中心的日常运作将由一名全职科学主任协调。印第安纳大学和生物化学与分子生物学系已承诺为科学主任提供资金支持，并用于Procise cLC蛋白质测序仪的维护。

This is an NIH-NCRR Shared Instrumentation Grant to purchase an ABI Procise 491 cLC protein sequencer for the Indiana University School of Medicine. We have a core of four NIH-funded user groups who will occupy 90 percent of the available instrument time. The titles of the projects are: 1) Genomic Approach to Determining Phosphorylation Targets for Protein Kinases, 2) Identification of the Phosphoprotein Phosphatases Responsible for Regulation of the Branched-Chain alpha-Ketoacid Dehydrogenase and Pyruvate Dehydrogenase Complexes, 3) Identification and Structural Characterization of Cardiac Sarcoplasmic Proteins Required for Calcium Uptake and Release, 4) Identification of retinoid metabolizing enzymes in hepatic stellate cells. There is an additional core of ten other NIH-funded users who have expressed an interest in protein sequencing and will occupy the remaining 10 percent of the instrument time. There is no generally available protein sequencer on the Medical School campus. The Procise cLC will be placed in the newly formed interdisciplinary Center for Structural Biology at Indiana University School of Medicine whose goal is to provide state-of-the-art instrumentation and faculty consultants for researchers interesting in characterizing the structural properties of biologic macromolecules. The day-to-day operations of the Center will be coordinated by a full-time Scientific Director. Indiana University and the Department of Biochemistry and Molecular Biology have committed funds for the support of the Scientific Director and for maintenance of the Procise cLC protein sequencer.