MALDI MASS SPECTROMETER FOR PROTEIN AND PEPTIDE ANALYSIS

用于蛋白质和肽分析的 MALDI 质谱仪

• 批准号: 6051121
• 负责人: OWEN W GRIFFITH
• 金额: $ 24.28万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-15 至 2001-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6051121
• 关键词: biomedical equipment purchase; matrix assisted laser desorption ionization

A matrix-assisted laser desorption ionization time-of-flight (MALDI- TOF) mass spectrometer is required for both ongoing and proposed studies to be carried out by approximately 20 NIH-funded investigators at the Medical College of Wisconsin and Blood Research Institute of Wisconsin. The proposed instrument will become part of the Protein and Nucleic Acid Shared Facility of the Medical College and will be operated in the Facility by Dr. Jasper Chu, an investigator with 5 years mass spectrometry experience characterizing proteins and peptides. The MALDI-TOF mass spectrometer will be used mainly for accurate and sensitive mass analysis and structure determination of proteins and peptides and of derivatives of proteins and peptides including covalent enzyme-inhibitor adducts, phosphorylated and glycosylated derivatives, and derivatives bearing modifications of N-terminal amino acids. The instrument will also be used for analysis of large protein complexes and for the identification of proteins from 2D gels. We have determined that the MALDI-TOF mass spectrometer system best meets these combined needs. Among its advantages are: (i) high mass range for proteins (>100kD), (ii) high sensitivity (fmol range), (iii) ability to sequence peptides using post-source decay spectra and "ladder sequencing," and (iv) ability to analyze complex sample mixtures.

由威斯康星州医学院和威斯康星州血液研究所的约20名NIH资助的研究者进行的正在进行的和拟定的研究都需要基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱仪。拟定的仪器将成为医学院蛋白质和核酸共享设施的一部分，并将由碧玉朱博士在该设施中操作，他是一名具有5年质谱分析蛋白质和肽特性经验的研究员。MALDI-TOF质谱仪将主要用于蛋白质和肽以及蛋白质和肽的衍生物（包括共价酶抑制剂加合物、磷酸化和糖基化衍生物以及具有N-末端氨基酸修饰的衍生物）的准确和灵敏的质量分析和结构测定。该仪器还将用于分析大型蛋白质复合物和从2D凝胶中鉴定蛋白质。我们已经确定MALDI-TOF质谱仪系统最能满足这些综合需求。其优点包括：（i）蛋白质的高质量范围（> 100 kD），（ii）高灵敏度（fmol范围），（iii）使用源后衰变谱和“梯状测序”对肽进行测序的能力，以及（iv）分析复杂样品混合物的能力。