3D Momentum Imaging of Matrix-Assisted Laser Desorption/Ionization (MALDI) in the Time Domain

时域基质辅助激光解吸/电离 (MALDI) 的 3D 动量成像

基本信息

  • 批准号:
    2107860
  • 负责人:
  • 金额:
    $ 45万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-09-01 至 2024-08-31
  • 项目状态:
    已结题

项目摘要

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, Professor Wen Li and his group at Wayne State University are undertaking fundamental studies related to mass spectrometry, a powerful method of chemical analysis with broad applications in biology, chemistry, and medicine. Specifically, the Li group is studying the mechanism of Matrix-Assisted Laser Desorption/Ionization (MALDI), a widely employed technique for producing ions that are subjected to mass analysis. Despite decades of research effort, knowledge of the details of the underlying mechanism of MALDI remains imperfect. This project aims to enhance our understanding of these details using newly developed research tools to provide unprecedented insights into the MALDI process. It is expected that improved insight may lead to improved sensitivity. Students engaged in these studies will gain excellent interdisciplinary training. Dr. Li is also active in outreach to high school students, in efforts to enhance their interest in career paths in science.The project aims to enhance understanding of the mechanisms of primary ionization in MALDI and aims to improve the sensitivity of the technique in detecting complex and large molecules of biological relevance. Femto- and picosecond lasers coupled with high-resolution three-dimensional momentum imaging of both ejected electrons and ions will be employed with the goal of providing unprecedented details about ionization dynamics. Schemes using ultrashort pulse pumping to reduce the sample loss to neutral desorption will be devised with an aim of significantly increasing sensitivity. Specifically, three projects will be integrated to unravel the complex dynamics: 1) developing a novel ultrafast thermometry method based on electron imaging with a resolution of tens of picoseconds for measuring the time-resolved temperatures of samples; 2) carrying out time-resolved thermometry measurements on DHB to assess the validity of various models; and 3) investigating a new laser desorption/ionization mechanism driven by femtosecond pulses. These studies are expected to illuminate details of the initial ionization process and may lead to enhancement of MALDI sensitivity.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
在美国韦恩州立大学化学系化学测量与成像项目的支持下,李文教授和他的团队正在进行与质谱相关的基础研究,质谱是一种强大的化学分析方法,在生物、化学和医学领域有着广泛的应用。具体来说,Li小组正在研究基质辅助激光解吸/电离(MALDI)的机制,这是一种广泛应用于生产离子的技术,可以进行质量分析。尽管经过数十年的研究,对MALDI潜在机制的细节了解仍然不完善。该项目旨在通过使用新开发的研究工具来提高我们对这些细节的理解,从而对MALDI过程提供前所未有的见解。预期洞察力的提高可能导致灵敏度的提高。参与这些研究的学生将获得优秀的跨学科训练。李博士还积极与高中学生接触,努力提高他们对科学职业道路的兴趣。该项目旨在加强对MALDI中初级电离机制的了解,并旨在提高该技术在检测生物相关的复杂和大分子方面的灵敏度。飞秒和皮秒激光结合高分辨率的三维动量成像,将提供电离动力学的前所未有的细节。方案使用超短脉冲泵浦,以减少样品损失的中性解吸将设计与显著提高灵敏度的目的。具体而言,将整合三个项目来解开复杂的动力学:1)开发一种基于分辨率为数十皮秒的电子成像的新型超快速测温方法,用于测量样品的时间分辨温度;2)对DHB进行时间分辨测温,评估各种模型的有效性;3)研究飞秒脉冲驱动的激光解吸/电离机制。这些研究有望阐明初始电离过程的细节,并可能提高MALDI的灵敏度。该奖项反映了美国国家科学基金会的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Three-dimensional (3D) moment imaging with a USB3 oscilloscope.
使用 USB3 示波器进行三维 (3D) 矩成像。
Picosecond vs Femtosecond: Are All Laser Desorption Ionizations Created Equal?
皮秒与飞秒:所有激光解吸电离作用都相同吗?
  • DOI:
    10.1021/acs.jpcc.2c06573
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Stewart, Gabriel A.;Debrah, Duke;Ranathunga, Yasashri;Olowolafe, Temitayo A.;Schlegel, H. Bernhard;Lee, Suk Kyoung;Li, Wen
  • 通讯作者:
    Li, Wen
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Wen Li其他文献

Transient Receptor Potential Channels and Chronic Airway Inflammatory Diseases: A Comprehensive Review
瞬时受体电位通道与慢性气道炎症性疾病:综合综述
  • DOI:
    10.1007/s00408-018-0145-3
  • 发表时间:
    2018-08
  • 期刊:
  • 影响因子:
    5
  • 作者:
    Yang Xia;Lexin Xia;Lingyun Lou;Rui Jin;Huahao Shen;Wen Li
  • 通讯作者:
    Wen Li
Self-reductive synthesis of MXene/Na0.55Mn1.4Ti0.6O4 hybrids for high-performance symmetric lithium ion batteries
用于高性能对称锂离子电池的MXene/Na0.55Mn1.4Ti0.6O4杂化物的自还原合成
  • DOI:
    10.1039/c9ta00744j
  • 发表时间:
    2019
  • 期刊:
  • 影响因子:
    11.9
  • 作者:
    Guodong Zou;Bingcheng Ge;Hao Zhang;Qingrui Zhang;Carlos Fern;ez;Wen Li;Jianyu Huang;Qiuming Peng
  • 通讯作者:
    Qiuming Peng
Construction of cuproptosis-related lncRNAs/mRNAs model and prognostic prediction of hepatocellular carcinoma
铜凋亡相关lncRNAs/mRNAs模型构建及肝细胞癌预后预测
  • DOI:
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Lingxue Tang;Tong Wang;Wen Li;Sheng Yu;Senbang Yao;Huaidong Cheng
  • 通讯作者:
    Huaidong Cheng
Implantable Parylene MEMS RF Coil for Epiretinal Prostheses
用于视网膜前假体的植入式聚对二甲苯 MEMS 射频线圈
  • DOI:
  • 发表时间:
    2012
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Wen Li;D. Rodger;J. Weiland;M. Humayun;Wentai Liu;Y. Tai
  • 通讯作者:
    Y. Tai
Synthesis and antitumor activity of 7-azaindirubin
7-氮杂靛红的合成及其抗肿瘤活性
  • DOI:
  • 发表时间:
    2009
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Zhao;Wen Li;Fu Li;Lei Zhang;W. Hua;Jingcai Cheng;Qizheng Yao
  • 通讯作者:
    Qizheng Yao

Wen Li的其他文献

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{{ truncateString('Wen Li', 18)}}的其他基金

Collaborative Research: SCH: A wireless optoelectronic implant for closed-loop control of bi-hormone secretion from genetically modified islet organoid grafts
合作研究:SCH:一种无线光电植入物,用于闭环控制转基因胰岛类器官移植物的双激素分泌
  • 批准号:
    2306708
  • 财政年份:
    2023
  • 资助金额:
    $ 45万
  • 项目类别:
    Standard Grant
NSF MRI: Acquisition of a Nanoscale 3D Printer for Medical Device Precision Manufacturing at Michigan State University
NSF MRI:密歇根州立大学采购用于医疗器械精密制造的纳米级 3D 打印机
  • 批准号:
    2216131
  • 财政年份:
    2022
  • 资助金额:
    $ 45万
  • 项目类别:
    Standard Grant
SitS: Wireless, sustainable, and automated sensory system for in-situ monitoring of soil heavy metals
SitS:用于土壤重金属原位监测的无线、可持续和自动化传感系统
  • 批准号:
    2226500
  • 财政年份:
    2022
  • 资助金额:
    $ 45万
  • 项目类别:
    Standard Grant
Probing Multi-Electron Dynamics with Absolute Carrier-Envelope-Phase (CEP) Dependent Strong Field Interaction
利用绝对载流子包络相位 (CEP) 相关的强场相互作用探测多电子动力学
  • 批准号:
    2012098
  • 财政年份:
    2020
  • 资助金额:
    $ 45万
  • 项目类别:
    Standard Grant
Collaborative Research: Causes and Consequences of Relativistic Electron Precipitation as Revealed by the CubeSat Mission ELFIN’s Pitch-Angle Resolved Loss Cone Measurements
合作研究:立方体卫星任务 ELFIN 的俯仰角解析损耗锥测量揭示的相对论电子沉淀的原因和后果
  • 批准号:
    2019950
  • 财政年份:
    2020
  • 资助金额:
    $ 45万
  • 项目类别:
    Standard Grant
Collaborative Research: NCS-FO: Intelligent Closed-Loop Neural Interface System for Studying Mechanisms of Somatosensory Feedback in Control of Functional and Stable Locomotion
合作研究:NCS-FO:智能闭环神经接口系统,用于研究体感反馈控制功能性和稳定运动的机制
  • 批准号:
    2024270
  • 财政年份:
    2020
  • 资助金额:
    $ 45万
  • 项目类别:
    Standard Grant
CAREER: Exploring Mysterious Whistler Mode Waves in Earth's Plasmaspheric Plumes
职业:探索地球等离子层羽流中神秘的惠斯勒模式波
  • 批准号:
    1847818
  • 财政年份:
    2019
  • 资助金额:
    $ 45万
  • 项目类别:
    Continuing Grant
RET Site: Multidisciplinary Computational Solutions to Smart Sensors and Sensing Systems
RET 站点:智能传感器和传感系统的多学科计算解决方案
  • 批准号:
    1854985
  • 财政年份:
    2019
  • 资助金额:
    $ 45万
  • 项目类别:
    Standard Grant
EAGER: Real-Time: Free-Floating Wireless Implantable Optical Stimulators for Untethered Optogenetics
EAGER:实时:用于不受限制的光遗传学的自由浮动无线植入式光学刺激器
  • 批准号:
    1923187
  • 财政年份:
    2019
  • 资助金额:
    $ 45万
  • 项目类别:
    Standard Grant
Collaborative Research: GEM: Modulation of Plasma Waves by Thermal Plasma Density Variation in the Inner Magnetosphere
合作研究:GEM:内磁层热等离子体密度变化对等离子体波的调制
  • 批准号:
    1723342
  • 财政年份:
    2017
  • 资助金额:
    $ 45万
  • 项目类别:
    Continuing Grant

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动量空间中的三重子及其动力学成像
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