Role of PKA Cbeta Subunits in Learning and Memory

PKA Cbeta 亚基在学习和记忆中的作用

• 批准号: 6338522
• 负责人: DOUGLAS G HOWE
• 金额: $ 4.02万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-05 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6338522
• 关键词: behavioral /social science research tag; biological signal transduction; cAMP response element binding protein; calmodulin dependent protein kinase; conditioning; enzyme activity; fear; gene expression; gene targeting; genetically modified animals; laboratory mouse; learning; memory; mitogen activated protein kinase; neurogenetics; protein isoforms; protein kinase A

DESCRIPTION: (Adapted from applicant's abstract): The proposed research involves the generation of mice lacking all C13 subunits of protein kinase A (PKA). These mice will be utilized in studies examining the role of this PKA subunit in learning and memory. PKA is an essential enzyme in the process of learning and memory, and the Cb subunit is expressed in regions of the brain known to be critical for the establishment of long-term memory. Mice lacking Cb will be tested in spatial and fear learning tasks to determine the significance of Cb in these types of memory. The molecular mechanism of learning and memory will be examined by determining the effect of knocking out Cb on signal transduction and gene expression events thought to be important for learning and memory. Finally, a transgenic approach will be utilized to attempt to rescue the phenotype of the Cb knockout mice. A detailed understanding of the molecular mechanism of learning and memory is necessary if we are to reduce, reverse or even prevent the memory loss associated with old age and neurological disease.

产品说明：（改编自申请人的摘要）：拟议的研究涉及产生缺乏蛋白激酶A（PKA）的所有C13亚基的小鼠。这些小鼠将用于研究PKA亚基在学习和记忆中的作用。PKA是学习和记忆过程中的必需酶，Cb亚基在已知对建立长期记忆至关重要的大脑区域中表达。将在空间和恐惧学习任务中测试缺乏Cb的小鼠，以确定Cb在这些类型的记忆中的重要性。将通过确定敲除Cb对信号转导和被认为对学习和记忆重要的基因表达事件的影响来检查学习和记忆的分子机制。最后，将利用转基因方法来尝试挽救Cb敲除小鼠的表型。如果我们要减少、逆转甚至预防与老年和神经系统疾病相关的记忆丧失，就必须详细了解学习和记忆的分子机制。