Highly-Multiplexed Capillary Electrophoresis

高度多重毛细管电泳

• 批准号: 6404464
• 负责人: HO-MING PANG
• 金额: $ 9.94万
• 依托单位: COMBISEP, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2002-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6404464
• 关键词: analytical chemistry; biomedical equipment development; capillary electrophoresis; computer program /software; high throughput technology; physical separation; technology /technique development

DESCRIPTION (provided by applicant): The explosive growth in biological, pharmaceutical, and human health screening technologies has challenged the analytical sciences to create methodologies for high-sample throughput. Of the many possible methodologies, capillary electrophoresis (CE) has an unrecognized potential to serve as a platform for a highly multiplexed analysis system that allows for extremely high sample throughput. However, CE traditionally suffers from unacceptably high sample-to-sample and capillary-to-capillary variations in elution profiles and injection volumes. This variability is one of the main impediments to the use of CE in routine analysis. Furthermore, today's multiplexed CE systems are based only on fluorescence read-out concepts, which are ineffective in detecting -90 percent of the likely target compounds. This project describes the design fabrication, and implementation of a 96-capillary array CE system that 1) employs an absorbance-based read-out methodology and 2) utilizes the near-continuous measurement, collection, and application of the current flow at each individual capillary. The former significantly expands the scope of the system. The latter uses the collected current for self-normalization of the electropherogram through application of the migration index, which can yield precisions that compare favorably with that of high performance liquid chromatography. Success in Phase I will lead to a Phase II program focused on optimizing the system and methods to test biological fluids to perform disease diagnosis and/or prognosis as well as combinatorial drug synthesis and activity screening. Phase II would accumulate in actual field tests being performed and compared to traditional, accepted methods. PROPOSED COMMERCIAL APPLICATION: The ultimate goal of the project for commercial applications is the creation of a packaged system that has proven capabilities for solving problems in the health-related applications (e.g., cancer gene detection, multation analysis, forensic genotyping and drug efficacy) and other areas demanding reliable, high throughput analysis.

描述(申请人提供)：生物科技的爆炸性增长 制药和人类健康筛查技术挑战了 分析科学为高样品吞吐量创造方法学。中的 有许多可能的方法学，毛细管电泳法(CE)有一种未被认可的 有可能成为高度多元化的分析系统的平台， 允许极高的样品吞吐量。然而，行政长官传统上受到 从不可接受的高样本到样本和毛细管到毛细管的差异 在洗脱曲线和进样量中。这种可变性是主要的 常规分析中使用毛细管电泳法的障碍。此外，今天的 多路CE系统仅基于荧光读出概念，其 在检测-90%的可能目标化合物方面无效。这 项目描述了96毛细管的设计、制造和实施 阵列CE系统，其1)采用基于吸光度的读出方法和2) 利用近乎连续的测量、收集和应用 电流在每个单独的毛细管上流动。前者极大地扩展了 系统的范围。后者将收集的电流用于 应用迁移法实现电泳图的自归一化 索引，它可以产生与High相媲美的精度 高效液相色谱。第一阶段的成功将导致第二阶段的成功 专注于优化测试生物体液的系统和方法的计划 进行疾病诊断和/或预后以及联合用药 合成和活性筛选。第二阶段将在实际领域中积累 正在进行的测试，并与传统的、公认的方法进行比较。 建议的商业应用： 该项目的最终目标是为商业应用程序创建一个打包的 具有解决健康相关应用程序中的问题的能力的系统 (例如，癌症基因检测、多菌种分析、法医基因分型和药物疗效) 以及其他需要可靠、高吞吐量分析的领域。