ROLLING CIRCLE AMPLIFICATION ANALYSIS OF CERVICAL CELLS

宫颈细胞滚圈扩增分析

• 批准号: 6287892
• 负责人: STEVEN P PICCOLI
• 金额: $ 9.99万
• 依托单位: MOLECULAR STAGING, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-10 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6287892
• 关键词: cervix neoplasms; diagnosis design /evaluation; human papillomavirus; messenger RNA; neoplasm /cancer diagnosis; neoplastic process; nucleic acid amplification techniques; tumor antigens; virus DNA; virus genetics; virus related neoplasm /cancer

A subset of human papillomavirus (HPV) types have been implicated in cervical cancer. The determination of the presence of the HPV types associated with cervical cancer may serve as adjunct to Pap smear screening of cervical samples. This analysis could reduce the incidence of invasive cervical cancer, while decreasing the human and economic costs associated with Pap smear screening and reflex histological analysis. HPV analysis could distinguish between transient infections that resolve without the development of high-grade cervical lesions, and the presence of rare cells that have been biologically altered by HPV, and are progressing from a localized intraepithelial lesion to invasive cancer. This project will develop a method using Rolling Circle Amplification to analyze individual cells within a cervical sample and establish the presence of HPV DNA and RNA sequences, and in Phase II, establish the relationship of other markers to the early stages of HPV-related cervical cancer. The majors goals of this project are to develop a means to detect rare HPV-transformed cells that are missed by conventional cytology and validate RCA protocols for molecular analyses of cells with respect to features of HPV gene expression that present a risk of neoplastic progression rather than a transient infection. PROPOSED COMMERCIAL APPLICATIONS: The successful completion of this project will provide two types of products. The first could serve as an adjunct Pap smears by providing an indication when a "second look" might reveal rare aberrant cervical cells missed by cytology. The ultimate product would enhance or replace the annual Pap smear by providing molecular information, predicting early cervical cancer lesions, that is not visible to a trained cytotechnologist or pathologist.

人乳头瘤病毒（HPV）类型的一个子集与宫颈癌有关。检测与宫颈癌相关的人乳头瘤病毒类型可作为子宫颈样本巴氏涂片筛查的辅助手段。该分析可以降低浸润性宫颈癌的发病率，同时降低与巴氏涂片筛查和反射组织学分析相关的人力和经济成本。HPV分析可以区分短暂性感染（未发展为高级别宫颈病变）和罕见细胞（已被HPV生物学改变，并从局部上皮内病变发展为浸润性癌症）。该项目将开发一种方法，使用滚动圈扩增来分析宫颈样本中的单个细胞，并确定HPV DNA和RNA序列的存在，并在第二阶段建立其他标志物与HPV相关宫颈癌早期阶段的关系。该项目的主要目标是开发一种方法来检测传统细胞学所遗漏的罕见的HPV转化细胞，并验证RCA方案用于细胞的分子分析，这些分析涉及HPV基因表达的特征，这些特征具有肿瘤进展的风险，而不是短暂的感染。建议的商业应用：该项目的成功完成将提供两类产品。第一次检查可以作为子宫颈抹片检查的辅助手段，当“第二次检查”可能会发现细胞学上没有发现的罕见异常宫颈细胞时，它可以提供指示。最终的产品将通过提供分子信息来增强或取代每年的子宫颈抹片检查，预测早期宫颈癌病变，这是训练有素的细胞技术专家或病理学家无法看到的。