OLIGONUCLEOTIDE MICROARRAYS FOR GENE EXPRESSION

应聘者？S描述：我们将开发一种基于喷墨技术的微点胶 用于以微阵列形式原位合成寡核苷酸的系统 基因表达研究(表达芯片)。多通道喷墨设备将 可用于将吞吐量提高一个数量级 每个基方法的分配器。基于喷墨的方法将：1)允许 寡核苷酸设计的灵活性，2)确保高通量制造 功能3)提供了一种商业上现有的低成本替代方案 现有的基于寡核苷酸的微阵列，以及4)在 可与商业上可用的荧光检测一起使用的格式 扫描仪/仪器。 微阵列将有助于发现药物靶点，这可能 与疾病期间表达的基因和/或基因产物相互作用 州政府。对这些药物先导的毒理学和药理学反应可以 也被监控。这种微芯片的使用将使医疗专业人员 开出最有效、副作用最少的药物 个体的基因表达谱。低成本的微阵列将有助于 将这项使能技术掌握在所有研究人员手中，从而 促进我们对细胞内基因表达变化的理解 对发育线索、生理或病理条件的反应，或 外部刺激。 建议的商业应用： 原位寡核苷酸合成器将提供低成本定制和现成 用于各种研究应用的微阵列。此类数组中的数据可用于构建 不同环境下细胞和组织基因表达谱生物信息学数据库 为客观衡量正常和疾病状态及疾病提供条件 关联的路径。基因表达微阵列也将提供必要的信息 对于正常状态和疾病状态的描述，导致了 新疗法。

APPLICANT?S DESCRIPTION: We will develop an ink-jet based microdispensing system for in situ synthesis of oligonucleotides in a microarray format for gene expression studies (expression chip). Multi-channel ink-jet devices will be utilized to increase throughput by an order of magnitude over single dispenser per base methods. The ink-jet based approach will: 1) allow flexibility in oligonucleotide design, 2) ensure high-throughput manufacture capabilities 3) provide a low cost alternative to current commercially available oligonucleotide-based microarrays, and 4) generate microarrays in a format that can be used with commercially available fluorescence detection scanners/instruments. The microarrays will facilitate the discovery of drug targets, which may interact with the genes and/or gene products expressed during the disease state. The toxicological and pharmacological responses to these drug leads can also be monitored. The use of such microchips will allow medical professionals to prescribe the most effective drugs, having the least side effects, based on a gene expression profile of an individual. Low cost microarrays will help to place this enabling technology into the hands of all researchers, thereby promoting our understanding of the changes in gene expression during cellular responses to developmental cues, physiological or pathological conditions, or external stimuli. PROPOSED COMMERCIAL APPLICATION: The in situ oligonucleotide synthesizer will provide low cost custom and "off-the-shelf" microarrays for various research applications. Data from such arrays can be used to build a bioinformatics database of gene expression profiles of cells and tissues under various conditions to provide for objective measurements of normal and disease states and disease associated pathways. Gene expression microarrays will also provide essential information for the characterization of normal versus diseased states, leading to the development of new treatment.