LC Microdispenser for High Resolution MALDI-TOF Analysis

用于高分辨率 MALDI-TOF 分析的 LC 微量分配器

• 批准号: 6951484
• 负责人: Patrick W Cooley
• 金额: $ 32.77万
• 依托单位: MICROFAB TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2006-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6951484
• 关键词: biomedical automation; biomedical equipment development; fungal proteins; high throughput technology; liquid chromatography; liquid chromatography mass spectrometry; mass spectrometry; matrix assisted laser desorption ionization; miniature biomedical equipment; proteomics

DESCRIPTION (provided by applicant): We will construct a LC Microdispenser system for rapid liquid chromatography and high throughput loading of digested protein samples onto MALDI-TOF MS targets. This system will incorporate automated preparation of the reversed phase column, including automatic sample loading and integration into the microdispensing device. The capacity of the multi-fluid print head will be increased to 20 different fluids to increase throughput for multiple samples and/or to allow for different chromatography treatments. This system will provide for the maximum conservation of sample volume combined with the rapid LC separation of peptides, resulting in improved MALDI-TOF MS analysis through a reduction in ion suppression. The function and sensitivity of the LC Microdispensor system will be verified by the detection and peptide mass fingerprinting of low abundance proteins in Saccharomyces cerevisiae. Commercialization efforts will be conducted in parallel with the research efforts, in collaboration with our commercialization partners: Proteome Systems, Ltd., Shimadzu Biotech, and Millipore Corporation. Commercial Applications: High throughput instrumentation that improves the sensitivity and dynamic range during MALDI-TOF MS and results in the peptide mass fingerprinting of low abundance proteins is highly desired in the rapidly developing field of proteomics. Details regarding protein expression and post-translational modifications can reveal processes within a biological system useful for drug discovery and the diagnosis and treatment of disease and cancer.

描述（由申请人提供）：我们将构建一个LC微分配器系统，用于快速液相色谱和将消化的蛋白质样品高通量加载到MALDI-TOF MS靶标上。该系统将包括反相柱的自动制备，包括自动样品加载和集成到微分配装置中。多流体打印头的容量将增加到20种不同的流体，以增加多个样品的吞吐量和/或允许不同的色谱处理。该系统将最大限度地节省样品体积，并结合肽的快速LC分离，从而通过减少离子抑制改善MALDI-TOF MS分析。LC微量分配器系统的功能和灵敏度将通过酿酒酵母中低丰度蛋白的检测和肽质量指纹图谱来验证。商业化工作将与研究工作同时进行，与我们的商业化合作伙伴合作：Proteome Systems，Ltd.，Shimadzu Biotech和Millipore Corporation。 商业应用： 在快速发展的蛋白质组学领域中，非常需要提高MALDI-TOF MS期间的灵敏度和动态范围并导致低丰度蛋白质的肽质量指纹的高通量仪器。关于蛋白质表达和翻译后修饰的细节可以揭示生物系统内用于药物发现以及疾病和癌症的诊断和治疗的过程。