Characterisation of key Bdellovibrio bacteriovorus predation proteins

关键噬菌弧菌捕食蛋白的表征

• 批准号: 1790996
• 金额: --
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1790996
• 关键词: Characterisation; key; Bdellovibrio; bacteriovorus; predation

The bacterial predator Bdellovibrio bacteriovorus is a useful, natural obligate killer of other bacteria. The potential exists to use Bdellovibrio (whole cells, or protein products in isolation) for a variety of antimicrobial purposes (healthcare, agriculture, bioremediation). We are beginning to investigate the means by which this specialized predator recognizes, enters and kills its prey.This project will use biochemistry and structural biology approaches to characterize proteins involved in predation. We will start by investigating three distinct sub-projects - (i) a novel protein proven to be essential for predation, postulated by us to be involved in protein folding; (ii) a receptor present in Bdellovibrio that contributes to killing efficiency, presumably by directing predator in a chemotactic fashion toward areas of high prey density; and (iii) a novel family of related proteins that regulate other genes during invasion of prey.

噬菌蛭弧菌（Bdellovibrio bacteriovorus）是一种有用的天然专性杀手。存在将蛭弧菌（全细胞或分离的蛋白质产品）用于各种抗微生物目的（医疗保健、农业、生物修复）的潜力。我们开始研究这种专门的捕食者识别、进入和杀死猎物的方法。本项目将使用生物化学和结构生物学方法来表征与捕食有关的蛋白质。我们将从研究三个不同的子项目开始-（i）一种新的蛋白质被证明是必不可少的捕食，我们假设参与蛋白质折叠;（ii）受体存在于蛭弧菌，有助于杀死效率，大概是通过引导捕食者在一个趋化的方式向高猎物密度的地区;和（iii）在猎物入侵期间调节其他基因的一个新的相关蛋白质家族。

项目成果

Two-site recognition of Staphylococcus aureus peptidoglycan by lysostaphin SH3b

• DOI: 10.1038/s41589-019-0393-4
• 发表时间: 2020-01-01
• 期刊: NATURE CHEMICAL BIOLOGY
• 影响因子: 14.8
• 作者: Gonzalez-Delgado, Luz S.;Walters-Morgan, Hannah;Mesnage, Stephane
• 通讯作者: Mesnage, Stephane