VIRUS CYTOKINE BINDING PROTEINS IN RAPID CYTOKINE ASSAYS

快速细胞因子测定中的病毒细胞因子结合蛋白

• 批准号: 6373777
• 负责人: Robert MARK Buller
• 金额: $ 30.89万
• 依托单位: RECEPTORPRO, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6373777
• 关键词: Poxviridae; binding proteins; biotechnology; cytokine; diagnosis design /evaluation; enzyme linked immunosorbent assay; immunologic assay /test; interferon gamma; method development; protein purification; protein structure function; technology /technique development

Cytokines are soluble protein mediators of immune function that are pivotal to a balanced innate or cell-mediated immune response. Since cytokine levels can be indicative of disease progression and/or resolution, and in a growing number of situations cytokines are being evaluated as therapeutics, there is a common need in both basic research and the health care industry to purify and/or measure key cytokines rapidly with accuracy, precision, and sensitivity, but at a low cost. Our long term goal is the replacement of antibodies in cytokine purification and immunoassays with cytokine binding proteins from microbial pathogens, which will be easier to manipulate for various applications and whose production by recombinant DNA technology should allow significant reductions in cost. Most importantly, the assays based on these cytokine binding proteins will be designed for rapid determination of cytokine levels which will be important in the diagnosis and/or monitoring of disease. To validate this approach we will study an IFN-gamma binding protein encoded by a murine poxvirus ectromelia. This protein is one of six known poxvirus cytokine binding proteins, and has the unique property of binding with high affinity both mouse and human IFN-gamma. PROPOSED COMMERCIAL APPLICATION: Virus cytokine binding proteins could be used to purify or to measure rapidly the therapeutically important cytokines with accuracy, precision and sensitivity, but at low cost.

细胞因子是免疫功能的可溶性蛋白质介导物，其对于平衡的先天或细胞介导的免疫应答是关键的。由于细胞因子水平可以指示疾病进展和/或消退，并且在越来越多的情况下，细胞因子正在被评估为治疗剂，因此在基础研究和医疗保健行业中存在以准确度、精确度和灵敏度快速纯化和/或测量关键细胞因子但成本低的共同需求。我们的长期目标是用来自微生物病原体的细胞因子结合蛋白替代细胞因子纯化和免疫测定中的抗体，这将更容易操作用于各种应用，并且其通过重组DNA技术的生产将允许显著降低成本。最重要的是，基于这些细胞因子结合蛋白的测定将被设计用于快速测定细胞因子水平，这在疾病的诊断和/或监测中将是重要的。为了验证这种方法，我们将研究由鼠痘病毒ectromelia编码的IFN-γ结合蛋白。该蛋白是六种已知的痘病毒细胞因子结合蛋白之一，并且具有以高亲和力结合小鼠和人IFN-γ的独特性质。拟定商业应用：病毒细胞因子结合蛋白可用于纯化或快速测定治疗上重要的细胞因子，具有准确性、精密度和灵敏度，但成本低。