Coenzyme Q and Aging in Caenorhabditis elegans
Coenzyme Q and Aging in Caenorhabditis elegans
批准号:
6491573
负责人:
CATHERINE FREITAG CLARKE
金额:
$10.0万
依托单位国家:
美国
项目类别:
财政年份:
2001
资助国家:
美国
项目状态:
已结题
起止时间:
2001-09-15 至 2002-03-31
中文摘要
描述:(由申请人提供)秀丽隐杆线虫是一种线虫
英文摘要
DESCRIPTION: (provided by applicant) The nematode Caenorhabditis elegans is an
excellent model for genetic studies of the aging process. Mutations in the
clk-1 gene of C. elegans result in an extended life span, slowed development
and sluggish behavior. CLK-l is a mitochondrial polypeptide with sequence and
functional conservation from humans to fungi. The yeast homologue of
CLK-l(Coq7p), is essential for the biosynthesis of ubiquinone (coenzyme Q, or
CoQ). The nematode, rat and human homologs of clk-1/COQ7 all function to
restore CoQ biosynthesis in the yeast coq7 null mutant. Our recent studies of
the C. elegans clk-l mutants suggest that the slowed rates of aging,
development and behavior are due to a biochemical defect in CoQ biosynthesis.
In the absence of dietary CoQ, (normally provided to nematodes by a diet of
CoQ-replete E. coli) the clk-l mutants display their true phenotype - growth
arrest in early development and sterility when emerging from the dauer stage.
The questions addressed in this proposal focus on the relationship between CoQ
and aging in the nematode model. The nature of the defect in the CoQ
biosynthetic pathway in the clk-l mutants will be defined. These studies will
make use of metabolic radiolabeling and analysis of total lipid extracts for
CoQ and CoQ-intermediates. C. elegans clk-1 mutants fed the CoQ-replete E. coli
rely on and assimilate this dietary CoQ. The regulation of uptake and
distribution of dietary CoQ is not understood in any system. C. elegans
provides a model for the assimilation and metabolism of exogenous CoQ in higher
organisms. A similar metabolism of exogenous CoQ exists in wild type nematodes.
The effect of dietary CoQ supplementation on the development, adult behavior
and life span will be evaluated in wild type nematodes, and in other cog
mutants of C. elegans. These studies will determine the effect of the clk-l
mutations on the biosynthesis of CoQ in the nematode system; a model that is
ideal for evaluating the relationship between coenzyme Q and aging. It is also
likely that these studies will indicate whether C. elegans can provide a
metazoan model uniquely suited to address questions regarding Q uptake,
metabolism and distribution.
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专著(0)
科研奖励(0)
会议论文
FUNCTIONAL ROLES OF COENZYME Q IN YEAST AND HUMAN CELLS
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批准号:7931073
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项目类别:
-
资助金额:$16.68万
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财政年份:2009
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负责人:CATHERINE FREITAG CLARKE
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依托单位:
FASEB Summer Research Conference on Biological Methylation
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批准号:7113505
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项目类别:
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资助金额:$3.13万
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财政年份:2006
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负责人:CATHERINE FREITAG CLARKE
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依托单位:
Coenzyme Q and Aging in Caenorhabditis elegans
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批准号:6625814
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项目类别:
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资助金额:$26.33万
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财政年份:2002
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负责人:CATHERINE FREITAG CLARKE
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依托单位:
Coenzyme Q and Aging in Caenorhabditis elegans
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批准号:6479157
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项目类别:
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资助金额:$32.49万
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财政年份:2002
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负责人:CATHERINE FREITAG CLARKE
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依托单位:
Coenzyme Q and Aging in Caenorhabditis elegans
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批准号:6719557
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项目类别:
-
资助金额:$26.33万
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财政年份:2002
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负责人:CATHERINE FREITAG CLARKE
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依托单位:
Coenzyme Q and Aging in Caenorhabditis elegans
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批准号:6876502
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项目类别:
-
资助金额:$26.33万
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财政年份:2002
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负责人:CATHERINE FREITAG CLARKE
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依托单位:
Coenzyme Q and Aging in Caenorhabditis elegans
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批准号:7031584
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项目类别:
-
资助金额:$25.71万
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财政年份:2002
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负责人:CATHERINE FREITAG CLARKE
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依托单位:
COENZYME Q AND AGING IN CAENORHABDITIES ELEGANS
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批准号:6129868
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项目类别:
-
资助金额:$7.65万
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财政年份:2000
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负责人:CATHERINE FREITAG CLARKE
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依托单位:
FUNCTIONAL ROLES OF UBIQUINONE IN YEAST AND HUMAN CELLS
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批准号:2183545
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项目类别:
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资助金额:$17.43万
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财政年份:1991
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负责人:CATHERINE FREITAG CLARKE
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依托单位:
FUNCTIONAL ROLES OF UBIQUINONE IN YEAST AND HUMAN CELLS
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批准号:2183546
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项目类别:
-
资助金额:$18.13万
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财政年份:1991
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负责人:CATHERINE FREITAG CLARKE
-
依托单位:
Functional roles of coenzyme Q in yeast and human cells
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批准号:6621520
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项目类别:
-
资助金额:$33.23万
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财政年份:1991
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负责人:CATHERINE FREITAG CLARKE
-
依托单位:
Functional roles of coenzyme Q in yeast and human cells
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批准号:7163840
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项目类别:
-
资助金额:$11.38万
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财政年份:1991
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负责人:CATHERINE FREITAG CLARKE
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依托单位:
FUNCTIONAL ROLES OF UBIQUINONE IN YEAST AND HUMAN CELLS
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批准号:2734695
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项目类别:
-
资助金额:$20.44万
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财政年份:1991
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负责人:CATHERINE FREITAG CLARKE
-
依托单位:
FUNCTIONAL ROLES OF COENZYME Q IN YEAST AND HUMAN CELLS
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批准号:7529887
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项目类别:
-
资助金额:$34.89万
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财政年份:1991
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负责人:CATHERINE FREITAG CLARKE
-
依托单位:
FUNCTIONAL ROLES OF UBIQUINONE IN YEAST AND HUMAN CELLS
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批准号:3305449
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项目类别:
-
资助金额:$15.69万
-
财政年份:1991
-
负责人:CATHERINE FREITAG CLARKE
-
依托单位:
FUNCTIONAL ROLES OF COENZYME Q IN YEAST AND HUMAN CELLS
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批准号:7142633
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项目类别:
-
资助金额:$24.8万
-
财政年份:1991
-
负责人:CATHERINE FREITAG CLARKE
-
依托单位:
FUNCTIONAL ROLES OF COENZYME Q IN YEAST AND HUMAN CELLS
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批准号:7373621
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项目类别:
-
资助金额:$34.93万
-
财政年份:1991
-
负责人:CATHERINE FREITAG CLARKE
-
依托单位:
Functional roles of coenzyme Q in yeast and human cells
-
批准号:6434774
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项目类别:
-
资助金额:$33.29万
-
财政年份:1991
-
负责人:CATHERINE FREITAG CLARKE
-
依托单位:
FUNCTIONAL ROLES OF UBIQUINONE IN YEAST AND HUMAN CELLS
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批准号:2398120
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项目类别:
-
资助金额:$20.48万
-
财政年份:1991
-
负责人:CATHERINE FREITAG CLARKE
-
依托单位:
FUNCTIONAL ROLES OF UBIQUINONE IN YEAST AND HUMAN CELLS
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批准号:3305450
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项目类别:
-
资助金额:$16.34万
-
财政年份:1991
-
负责人:CATHERINE FREITAG CLARKE
-
依托单位:
海外基金