IMPROVED VECTORS FOR THERAPEUTIC PROTEIN PRODUCTION

随着我们对越来越多的分泌性天然肽的功能的阐明，潜在的生物活性蛋白的多样性不断增加。此外，单链抗体显示出作为多种疾病状态的治疗剂的前景。这种潜在的有前途的蛋白质疗法的丰富性要求用于生产蛋白质的方法的改进，以满足动物中的功效试验和临床使用的需求。我们最近设计了一种基于修饰的牛乳头状瘤病毒（BPV）复制子的附加体载体，其可以在多种细胞类型（包括目前用于大规模蛋白质生产的细胞类型）中作为稳定的附加体维持。在本申请中，我们提出了将该载体改造用于生产治疗性血浆蛋白。拟议商业应用：不可用

The diversity of potential biotherapeutic proteins is continuously rising as we elucidate the function of an ever-increasing number of secreted natural peptides. In addition, single-chain antibodies are showing promise as therapeutic agents for a variety of disease states. This profusion of potentially promising protein therapies demands improvements in the methods used for producing proteins on the scale necessary to meet demands for efficacy trials in animals and for use in the clinic. We have recently devised an episomal vector based upon a modified bovine papilloma virus (BPV) replicon that can be maintained as a stable episome in a wide variety of cell types, including those currently used for large-scale protein production. In this application, we propose the adaptation of this vector for use in the manufacture of therapeutic plasma proteins. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE