Why do cells gorge themselves (Understanding the molecular mechanisms of chaperone mediated autophagy).

为什么细胞会自我吞噬（了解分子伴侣介导的自噬的分子机制）。

• 批准号: 1792461
• 金额: --
• 依托单位: University of York
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1792461
• 关键词: Why; do; cells; gorge; themselves

The fundamental cell processes that govern life begin to fail as we age resulting in diseases associated with old age such as cancer and neurodegeneration. One process that regulates cellular homeostasis is autophagy where cells 'eat themselves' to recycle nutrients and clear away unwanted proteins. This PhD project is specifically looking at a type of autophagy called chaperone mediated autophagy (CMA). The physiological function of CMA is to maintain energy homeostasis and protein quality control with CMA being important in neurodegeneration, cancer and aging. The project will take an inter-disciplinary approach where the molecular mechanisms of CMA will be investigated in the Pryor lab, who researches lysosomes, using mammalian cell culture and cell biology techniques (western blotting, molecular biology, confocal imaging, protein-protein interactions) and whole organism biology in the Sweeney lab, a Drosophila neurobiologist, using fruit flies to identify further the molecular components of CMA and the role of CMA in ageing.

随着年龄的增长，控制生命的基本细胞过程开始失灵，导致癌症和神经退化等与老年有关的疾病。调节细胞稳态的一个过程是自噬，细胞“吃掉自己”来回收营养物质，清除不需要的蛋白质。这个博士项目专门研究一种叫做伴侣介导的自噬（CMA）的自噬。CMA的生理功能是维持能量稳态和控制蛋白质质量，在神经退行性疾病、癌症和衰老中起重要作用。该项目将采用跨学科的方法，其中CMA的分子机制将在Pryor实验室进行研究，Pryor实验室研究溶酶体，使用哺乳动物细胞培养和细胞生物学技术（western blotting，分子生物学，共聚焦成像，蛋白质-蛋白质相互作用），而在Sweeney实验室，果蝇神经生物学家，使用果蝇进一步确定CMA的分子成分和CMA在衰老中的作用。