SENSORY PROCESSING BY SUBFORNICAL ORGAN IN BODY FLUID/CARDIOVASCULAR HOMEOSTASIS

体液和心血管稳态取决于摄食行为 以及调节肾水和钠损失的反射， 重新分配血流为了使适当的效应器机制 维持体液平衡和心血管功能， 在参与时，大脑必须接收有关当前状态的信息。 身体水分和血压。几个内脏感觉系统 将这种类型的信息提供给中枢神经系统（CNS）。这 输入是通过传入神经通路进行的， 循环因子在某些缺乏血脑的脑区 屏障大脑的三个可渗透区域接收血液传播的信号 被描述为感觉室周器官（CVOs）， 以穹窿下器官（SFO）为代表。 本建议的重点是证券及期货条例及其作用，在侦查流通 血管紧张素II，这些信息如何在这个结构中处理， 最终转移到与行为相关的前脑区域 以及对水矿物质平衡和血压的反射控制。本 该建议建立在我们先前对中央感觉和 涉及体液和心血管的传入信号的处理 体内平衡本研究的目的是探讨其机制 的感觉传递和神经处理与SFO的反应， 血液传播的血管紧张素II，然后表征传递这一途径 信息传递到特定的前脑核团 心血管和体液平衡。这些研究将在 体内和体外方法，以电生理学， 用SFO解剖学表征所识别的细胞。将细胞 分析以了解它们用于内部和内部的机制， 细胞信号从对这一重要问题的研究中获得的新信息 连接体循环和中枢神经系统的感觉结构 适当的有相关的理解正常的生理， 与体液失调状态相关的病理生理学 心血管调节，如高血压。

Body fluid and cardiovascular homeostasis depends on ingestive behaviors and on reflexes acting to modulate renal water and sodium loss and to redistribute blood flow. In order for the appropriate effector mechanisms that maintain body fluid balance and cardiovascular function to be engaged, the brain must receive information about the current status of body hydration and blood pressure. Several visceral sensory systems provide this type of information to the central nervous system (CNS). This input is carried over afferent neural pathways and by the direct action of circulating factors on select brain regions which lack a blood-brain barrier. Three permeable areas of the brain receiving blood-borne signals have been described as sensory circumventricular organs (CVOs) and are typified by the subfornical organ (SFO). This proposal focuses on the SFO and its role in detecting circulating angiotensin II, how this information is handled within this structure and ultimately transferred into forebrain regions involved in the behavioral and reflex control of hydromineral balance and blood pressure. The present proposal builds upon our prior studies of the central sensing and processing of afferent signals involved in body fluid and cardiovascular homeostasis. The aims of this research are to investigate the mechanisms of sensory transfer and neural processing with the SFO in response to blood-borne ANG II and then to characterize the pathways which convey this information to specific forebrain nuclei involved in maintaining cardiovascular and body fluid homeostasis. The studies will employ both in vivo and in vitro methods to electrophysiologically, pharmacologically and anatomically characterize identified cells with the SFO. The cells will be analyzed to understand the mechanisms they use for both intra- and inter- cellular signaling. New information derived from studies on this important sensory structure that couples the systemic circulation with the CNS proper has relevance for understanding both normal physiology and pathophysiology associated with states of disordered body fluid and cardiovascular regulation, such as in hypertension.