IN VIVO CONDITIONAL SIGNALING IN AVIAN DEVELOPMENT

禽类发育中的体内条件信号传导

• 批准号: 6530554
• 负责人: EDWARD LAUFER
• 金额: $ 8.22万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-09 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6530554
• 关键词: Aves; DNA replication; alpharetrovirus; bioengineering /biomedical engineering; chick embryo; chimeric proteins; drug administration rate /duration; embryology; fluorescence; functional /structural genomics; gene expression; gene targeting; genetic manipulation; green fluorescent proteins; immunocytochemistry; ligands; nuclear receptors; peptidylprolyl isomerase; posttranslational modifications; protein structure function; protein transport; technology /technique development; tissue /cell culture; transfection /expression vector; vertebrate embryology

The avian embryo is a powerful experimental system for exploring the tissue interactions and molecular mechanisms underlying vertebrate development. The combination of tissue transplantation methodologies and retrovirus-mediated ectopic gene expression or direct recombinant protein application have allowed enormous advances in the past few years. These experimental approaches are limited, however, by an inability to regulate the activity of the introduced gene or protein. In the experiments described in this proposal, the Laufer lab will adapt two conditional signaling systems for use in the avian system. The LBD (hormone receptor ligand binding domain) system uses mutant ligand binding domains of hormone receptors to reversibly inactivate constitutively activate protein: LBD fusions. Both the estrogen and progesterone receptor LBDs will be used. These are regulated by 4- hydroxy-tamoxifen and RU486, respectively. The FK506 binding protein (FKBP) system uses modular FKBP binding domains fused to partner proteins, and a bivalent synthetic ligand (AP20187) to regulate protein activity. Reagents will be developed using each system, and their properties investigated in vitro. Avian retroviruses expressing nuclear GFP:LBD fusions will be constructed, and ligand-dependent cytoplasmic to nuclear translocation used as a monitor of activation. Retroviruses that express a truncated FGF receptor: FKBP fusion protein will also be constructed; activation is caused by ligand-dependent dimerization. Activation in cell culture will be assessed through receptor phosphorylation and induction of DNA synthesis. For each of these reagents, activation kinetics and dose responses will be determined. The in vivo regulatory parameters of each fusion protein-ligand pair will then be established. Chick embryos will be infected with the recombinant retroviruses, and after allowing for expression of the target protein, embryos will be treated with the appropriate drug. nGFP:LBD activation will be determined via subcellular localization. FGFR activity will be assessed by FGF-dependent target gene expression using non-radioactive in situ hybridization histochemistry and induction of tissue growth. Various drug doses and application regimens (via superficial vasculature, intravenous or intralumenal injection) will determine the most efficacious means of target activation, as well as kinetics and dose responses. Together these systems will provide the means to regulate protein function in a spatial, temporal and quantitative fashion during avian development.

鸟类胚胎是研究脊椎动物发育过程中组织相互作用和分子机制的有力实验系统。组织移植方法与逆转录病毒介导的异位基因表达或直接重组蛋白应用的结合在过去几年中取得了巨大的进展。然而，由于无法调节引入的基因或蛋白质的活性，这些实验方法受到限制。在本提案中描述的实验中，劳弗实验室将采用两种条件信号系统用于鸟类系统。LBD（激素受体配体结合域）系统利用激素受体的突变配体结合域可逆地灭活组成型激活蛋白：LBD融合体。雌激素受体和孕激素受体lbd都将被使用。这些分别由4-羟基他莫昔芬和RU486调节。FK506结合蛋白（FKBP）系统使用模块化的FKBP结合域与伴侣蛋白融合，以及二价合成配体（AP20187）来调节蛋白质活性。将使用每种系统开发试剂，并在体外研究其性质。禽逆转录病毒表达核GFP:LBD融合将被构建，和配体依赖的细胞质到核的易位被用作激活的监测。还将构建表达截断的FGF受体FKBP融合蛋白的逆转录病毒；激活是由配体依赖的二聚化引起的。细胞培养中的激活将通过受体磷酸化和诱导DNA合成来评估。对于每一种试剂，将确定其活化动力学和剂量反应。然后建立每个融合蛋白-配体对的体内调控参数。鸡胚将被重组逆转录病毒感染，在允许目标蛋白表达后，胚胎将被适当的药物处理。nGFP:LBD的激活将通过亚细胞定位来确定。FGFR活性将通过使用非放射性原位杂交组织化学和诱导组织生长的FGFR依赖性靶基因表达来评估。不同的药物剂量和应用方案（通过浅表血管、静脉或腔内注射）将决定最有效的靶激活方式，以及动力学和剂量反应。这些系统将共同提供在鸟类发育过程中以空间、时间和数量方式调节蛋白质功能的手段。